线粒体质子传导率和H⁺/O比率与分离的肝细胞中的电子传递速率无关。
Mitochondrial proton conductance and H+/O ratio are independent of electron transport rate in isolated hepatocytes.
作者信息
Porter R K, Brand M D
机构信息
Department of Biochemistry, University of Cambridge, U.K.
出版信息
Biochem J. 1995 Sep 1;310 ( Pt 2)(Pt 2):379-82. doi: 10.1042/bj3100379.
Abstract
In this paper we examine the non-linearity of the relationship between the proton electrochemical gradient across the mitochondrial inner membrane (delta p) and oxygen consumption of non-phosphorylating mitochondria in situ in hepatocytes. Models proposing to explain the non-linear relationship were tested experimentally. It was shown that the mitochondrial proton conductance and the number of protons pumped to the cytosolic side of the mitochondrial inner membrane by the electron transport complexes per oxygen atom consumed (H+/O ratio) are independent of electron transport rate in mitochondria in isolated hepatocytes. The non-linearity of the plot of delta p against the non-phosphorylating oxygen consumption is due to either a potential-dependent slippage of the proton pumps of the mitochondrial inner membrane and/or a potential-dependent leakage of protons back across the mitochondrial inner membrane.
摘要
在本文中，我们研究了跨线粒体内膜的质子电化学梯度（δp）与肝细胞中非磷酸化线粒体原位氧消耗之间关系的非线性。对提出解释这种非线性关系的模型进行了实验测试。结果表明，线粒体质子电导以及每消耗一个氧原子时电子传递复合物泵送到线粒体内膜胞质侧的质子数（H⁺/O比率）与分离的肝细胞中线粒体的电子传递速率无关。δp与非磷酸化氧消耗关系图的非线性是由于线粒体内膜质子泵的电位依赖性滑动和/或质子跨线粒体内膜的电位依赖性泄漏。
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