Sokol L, Garber C, Shults M, Updike S
Clin Chem. 1980 Jan;26(1):89-92.
We present a rate-determination method for analyzing glucose. A glucose enzyme electrode serves as the sensor and is made by placing a gel-immobilized layer of glucose oxidase over the tip of a Clark-type O2 electrode. The electrode membrane is made of Teflon and is derivatized by etching with a suspension of colloidal sodium metal in organic solvent. The enzyme is coupled to the membrane surface by use of paraformaldehyde. The immobilized-enzyme method is compared with a similar solution-enzyme method and with the National Glucose Reference method. The immobilized enzyme method compares favorably with the solution-enzyme method and offers the advantages of simplicity, economy of enzyme, and linearity over a greater range of concentration.
我们提出了一种用于分析葡萄糖的速率测定方法。葡萄糖酶电极用作传感器，它是通过在克拉克型氧电极尖端放置一层凝胶固定化的葡萄糖氧化酶制成的。电极膜由聚四氟乙烯制成，并通过用胶体金属钠在有机溶剂中的悬浮液蚀刻进行衍生化处理。酶通过多聚甲醛与膜表面偶联。将固定化酶法与类似的溶液酶法以及国家葡萄糖参考方法进行了比较。固定化酶法与溶液酶法相比具有优势，并且具有操作简单、酶经济以及在更大浓度范围内具有线性的优点。
