Bernhard M I, Pace R C, Unger S W, Wanebo H J
J Immunol. 1980 Feb;124(2):964-8.
The blastogenic responsiveness of peripheral blood lymphocytes is a commonly applied measure of immune response capability. This assay is generally run under conditions of maximum lymphocyte stimulation. The purpose of this study was to evaluate which conditions of lymphocyte culture were able to best discriminate normal from subnormal lymphocyte responses to mitogens and antigens. Mitogen or antigen concentrations and the length of incubation time were varied while other assay conditions (serum supplement, lymphocyte concentration, amount of label, and length of label pulse) were kept constant. A total of 24 cancer patients and 25 normal controls were studied. Under optimal assay conditions that provided for maximum lymphocyte stimulation, we were unable to distinguish differences in response between young and old individuals or cancer patients and normal controls. However, at suboptimal mitogen/antigen concentrations or suboptimal incubation times, significant differences were observed in these populations. It is suggested that in the evaluation of lymphocyte stimulation responses in various clinical groups, suboptimal lymphocyte culture conditions may provide for maximum discrimination among the groups.
外周血淋巴细胞的增殖反应性是一种常用的免疫反应能力测量指标。该检测通常在淋巴细胞最大刺激条件下进行。本研究的目的是评估哪种淋巴细胞培养条件能够最佳地区分正常与低于正常水平的淋巴细胞对有丝分裂原和抗原的反应。在保持其他检测条件（血清补充、淋巴细胞浓度、标记量和标记脉冲时长）不变的情况下，改变有丝分裂原或抗原浓度以及孵育时间长度。共研究了24例癌症患者和25例正常对照。在提供最大淋巴细胞刺激的最佳检测条件下，我们无法区分年轻人与老年人或癌症患者与正常对照之间的反应差异。然而，在有丝分裂原/抗原浓度次优或孵育时间次优的情况下，在这些人群中观察到了显著差异。建议在评估不同临床组的淋巴细胞刺激反应时，次优的淋巴细胞培养条件可能会在各组之间提供最大的区分度。
