Davenport J W, McCarty R E
Biochim Biophys Acta. 1980 Feb 8;589(2):353-7. doi: 10.1016/0005-2728(80)90051-1.
The onset of photophosphorylation was determined by exposing chloroplast thylakoids to either single or multiple light flashes of varying duration. In aggreement with the results of Ort et al. (Ort, D.R., Dilley, R.A. and Good, N.E. (1976) Biochim. Biophys. Acta 449, 108--124), the permeant buffer imidazole in the presence of valinomycin and K+ did not greatly delay the onset of phosphorylation driven by multiple activation. In single flashes, however, the lag in the development of phosphorylation was much longer and imidazole caused a further delay. A significant delta pH was generated by the multiple flash regime. The onset of photophosphorylation is, therefore, consistent with the rise in transmembrane delta pH.
通过将叶绿体类囊体暴露于不同持续时间的单次或多次闪光来确定光磷酸化的起始。与奥尔特等人（奥尔特，D.R.，迪利，R.A.和古德，N.E.（1976年）《生物化学与生物物理学报》449卷，108 - 124页）的结果一致，在缬氨霉素和钾离子存在下的渗透性缓冲液咪唑不会显著延迟由多次激活驱动的磷酸化起始。然而，在单次闪光中，磷酸化发展的延迟要长得多，并且咪唑会导致进一步延迟。多次闪光模式产生了显著的跨膜pH差值。因此，光磷酸化的起始与跨膜pH差值的升高一致。
