Vinkler C, Avron M, Boyer P D
J Biol Chem. 1980 Mar 25;255(6):2263-6.
Permeant buffers (pyridine, imidazole, or phosphate) caused similar increases in the time required for onset of ATP synthesis in the light in the presence of valinomycin and K+; and in the illumination time required for postillumination phosphorylation with or without or without valinomycin and K+. Based on prior evidence, the minimum illumination time required for postillumination phosphorylation in thylakoid membranes is taken as a measure of the time required for formation of a transmembrane pH gradient sufficient to drive ATP synthesis. Our results are consistent with the view that, following illumination, as the transient transmembrane electric gradient decays, the establishment and maintenance of a pH gradient serves for energy transfer from the photosystems to the ATP synthase complex.
渗透性缓冲液（吡啶、咪唑或磷酸盐）在缬氨霉素和钾离子存在的情况下，会使光照下ATP合成开始所需的时间出现类似增加；并且在有或没有缬氨霉素和钾离子的情况下，会使光照后磷酸化所需的光照时间增加。基于先前的证据，类囊体膜中光照后磷酸化所需的最短光照时间被用作形成足以驱动ATP合成的跨膜pH梯度所需时间的衡量标准。我们的结果与以下观点一致：光照后，随着瞬时跨膜电势梯度衰减，pH梯度的建立和维持用于将能量从光系统转移到ATP合酶复合体。
