Shepherd J, Packard C J, Stewart J M, Vallance B D, Lawrie T D, Morgan H G
Clin Chim Acta. 1980 Feb 14;101(1):57-62. doi: 10.1016/0009-8981(80)90055-8.
High density lipoprotein subfractions (HDL2 and HDL3) were separated from the plasma of 25 healthy volunteers (13 males, 12 females) rate zonal ultracentrifugation. The rotor elution profile, measured at 280 nm, was used with the specific extinction coefficient for each subfraction (HDL2, 0.60 +/- 0.11 mg protein/A280nm, HDL3, 0.86 +/- 0.10 mg protein/A280nm (n=25) to calculate their plasma concentration. Their protein and lipid composition were also determined by chemical analysis. Plasma lipids, lipoprotein cholesterol, apolipoprotein A-I, apolipoprotein A-II and apolipoprotein B levels were measured in the same subjects and correlated with the HDL subfraction concentrations. HDL cholesterol and apolipoprotein A-I concentrations correlated significantly (p less than 0.01 and 0.02 respectively) with plasma HDL2, but not with HDL3. Indeed, the significantly higher levels of HDL cholesterol and apolipoprotein A-I in the female group could be attributed entirely to an increase in circulating HDL2. This data supports the proposal that the latter subfraction is the major contributor to the anti-atherogenic role of plasma HDL.
通过速率区带超速离心法从25名健康志愿者（13名男性，12名女性）的血浆中分离出高密度脂蛋白亚组分（HDL2和HDL3）。在280nm处测量的转子洗脱曲线，结合每个亚组分的特异性消光系数（HDL2，0.60±0.11mg蛋白/A280nm，HDL3，0.86±0.10mg蛋白/A280nm（n = 25））来计算它们在血浆中的浓度。它们的蛋白质和脂质组成也通过化学分析来确定。在相同受试者中测量血浆脂质、脂蛋白胆固醇、载脂蛋白A-I、载脂蛋白A-II和载脂蛋白B水平，并与HDL亚组分浓度进行相关性分析。HDL胆固醇和载脂蛋白A-I浓度与血浆HDL2显著相关（分别为p<0.01和0.02），但与HDL3不相关。实际上，女性组中HDL胆固醇和载脂蛋白A-I水平显著较高完全可归因于循环HDL2的增加。该数据支持了以下观点，即后一个亚组分是血浆HDL抗动脉粥样硬化作用的主要贡献者。
