Guillemin M P, Bauer D
Am Ind Hyg Assoc J. 1978 Nov;39(11):873-9. doi: 10.1080/0002889778507877.
Human volunteers were exposed in an experimental chamber to styrene (4 or 8 hours at 40 to 200 ppm) in order to obtain a quantitative relationship between exposure and urinary elimination of the metabolites mandelic and phenylglyoxylic acids (MA and PGA). For the analysis of PGA a new GC-method was used, based on reductive transformation of the relatively instable PGA into MA, which is stable enough for shipping and handling until final processing. The analysis of the post-exposure elimination shows that spot urine sampled in the morning after exposure and analysed for the sum of MA and PGA is the most reliable index for reflecing a preceding exposure to styrene.
将人类志愿者置于实验舱中，使其接触苯乙烯（40至200 ppm，持续4或8小时），以获取接触量与代谢产物扁桃酸和苯乙醛酸（MA和PGA）尿排泄量之间的定量关系。对于PGA的分析，采用了一种新的气相色谱法，该方法基于将相对不稳定的PGA还原转化为MA，MA足够稳定，便于运输和处理直至最终检测。暴露后消除情况的分析表明，接触后次日早晨采集的晨尿样本，分析其中MA和PGA的总量，是反映先前接触苯乙烯情况的最可靠指标。
