Hill S A, Denekamp J
Br J Radiol. 1978 Dec;51(612):997-1002. doi: 10.1259/0007-1285-51-612-997.
The effect of occluding the blood supply to a mouse tumour (with a metal clamp) has been studied for both irradiation and heating. Local heat was applied by immersion in a water bath for one hour at 42.8 degrees C or for 15 minutes at 44.8 degrees C. Occlusion of the blood supply during heating has a profound cytotoxic effect on the tumour, even in the absence of irradiation. Most tumours treated with 42.8 degrees C for one hour under clamped conditions were locally controlled whether they were irradiated or not. Tumours heated with their blood supply unobstructed showed a lesser sensitivity to heat, seen as an increased sensitivity to X rays with a thermal enhancement ratio of 1.8--2.6. With the shorter period of more intense heat (44.8 degrees C for 15 min), the effect of increasing the clamping time before heating was studied. The proportion of tumours locally controlled increased from 33% if the clamp was applied immediately before heating to 83% if the clamp was present for 60 minutes before heating commenced. No cures were observed for heat applied immediately before clamping, or immediately after release of the clamp. Accumulation of metabolic products or pH changes are implicated as the factors which alter the thermal sensitivity of these tumour cells.
对于照射和加热两种情况，均已研究了用金属夹阻断小鼠肿瘤血液供应的效果。通过将其浸入42.8摄氏度的水浴中1小时或44.8摄氏度的水浴中15分钟来施加局部加热。加热过程中阻断血液供应对肿瘤具有深远的细胞毒性作用，即使在没有照射的情况下也是如此。在夹住条件下于42.8摄氏度处理1小时的大多数肿瘤，无论是否接受照射，均实现了局部控制。血液供应未受阻的情况下加热的肿瘤对热的敏感性较低，表现为对X射线的敏感性增加，热增强比为1.8 - 2.6。对于较短时间的更强加热（44.8摄氏度15分钟），研究了在加热前增加夹住时间的效果。局部控制的肿瘤比例从加热前立即夹住时的33%增加到加热开始前夹住60分钟时的83%。在夹住前立即加热或夹住松开后立即加热均未观察到治愈情况。代谢产物的积累或pH值变化被认为是改变这些肿瘤细胞热敏感性的因素。
