磷酸二酯酶抑制剂己酮可可碱对人类精子活力的影响。

Effect of the phosphodiesterase inhibitor Pentoxyfylline on human sperm motility.

作者信息

Aparicio N J, de Turner E A, Schwarzstein L, Turner D

出版信息

Andrologia. 1980 Jan-Feb;12(1):49-54. doi: 10.1111/j.1439-0272.1980.tb00577.x.

DOI:10.1111/j.1439-0272.1980.tb00577.x

Abstract

Tyrode fluid and Tyrode fluid plus Pentoxifylline were individually added to aliquots of semen samples obtained from 6 normal men and 6 infertile patients considered to have idiopathic normogonadotropic oligoasthenozoospermia. Pentoxifylline was added to final concentrations of 0.15, 0.30 and 0.60 mM. One aliquot with no addition served as control. Samples were incubated in 37 degrees C and observed by light microscopy at 30 minutes and at 1, 2 and 4 hours after obtaining the material. At observation time, semen quality was evaluated by determining the percentages of forwardly progressive spermatozoa, slowly progressive spermatozoa, "in situ" motile spermatozoa, live and non-motile spermatozoa and dead spermatozoa. Results reported included only the first and last category. Tyrode fluid did not affect significantly the motility and the duration of activity of spermatozoa. Ejaculated human spermatozoa both from normal and asthenozoospermic men added the Pentoxifylline at 0.30 and 0.60 mM showed a longer lasting activity than those of control semen and semen added only with Tyrode fluid.

摘要

将台氏液和添加了己酮可可碱的台氏液分别加入到从6名正常男性和6名被认为患有特发性正常促性腺激素性少弱精子症的不育患者获取的精液样本等分试样中。己酮可可碱的最终添加浓度分别为0.15 mM、0.30 mM和0.60 mM。一份未添加任何物质的等分试样用作对照。样本在37℃下孵育，并在获取样本后30分钟以及1、2和4小时通过光学显微镜进行观察。在观察时间点，通过测定向前运动精子、缓慢运动精子、“原地”活动精子、活的和不活动精子以及死精子的百分比来评估精液质量。报告的结果仅包括第一类和最后一类。台氏液对精子的活力和活动持续时间没有显著影响。来自正常男性和弱精子症男性的射出人类精子，添加0.30 mM和0.60 mM己酮可可碱后，其活动持续时间比对照精液和仅添加台氏液的精液更长。