Devereux D F, Triche T J, Webber B L, Thibault L E, Brennan M F
Cancer. 1980 Jun 1;45(11):2811-5. doi: 10.1002/1097-0142(19800601)45:11<2811::aid-cncr2820451116>3.0.co;2-7.
The cause of the reduced wound breaking strength (WBS) in adriamycin-treated animals is unknown. Differential staining of histologic sections, collagen fiber diameter measurements by electron microscopy (EM), induction of maturation, and hydroxyproline content analysis were used to examine this defect. Differential staining and formaldehyde induction of maturation revealed no differences between mature and immature collagen content in scars of adriamycin-treated and control animals. Mean collagen fiber diameters in control rats were 76 +/- 27 nm and, in adriamycin treated rats, 62 +/- 10 nm (mean +/- SD) at 21 days. There was no difference in total hydroxyproline content between ADR and C animals. However, significant differences existed between the specific activity of new or 3H-hydroxyproline (scar collagen) at 14 days (controls, 372 +/- 9 cpm; adriamycin-treated, 129 +/- 5 cpm) and at 21 days (controls, 365 +/- 11 cpm; adriamycin treated, 172 +/- 14 cpm; mean +/- SEM; P less than 0.001). It appears that the defect contributing to reduced WBS in adriamycin treated animals is not due to a collagen maturation defect but rather to a reduction in scar collagen accumulation as measured by new hydroxyproline content and reduced fiber diameter as determined by EM measurements.
阿霉素处理的动物伤口抗张强度（WBS）降低的原因尚不清楚。采用组织学切片的差异染色、电子显微镜（EM）测量胶原纤维直径、诱导成熟以及羟脯氨酸含量分析来研究这一缺陷。差异染色和甲醛诱导成熟显示，阿霉素处理的动物和对照动物瘢痕中成熟和未成熟胶原含量无差异。21天时，对照大鼠的平均胶原纤维直径为76±27nm，阿霉素处理的大鼠为62±10nm（平均值±标准差）。阿霉素处理组（ADR）和对照组（C）动物的总羟脯氨酸含量没有差异。然而，14天时新合成的或3H-羟脯氨酸（瘢痕胶原）的比活性存在显著差异（对照组，372±9cpm；阿霉素处理组，129±5cpm），21天时也有显著差异（对照组，365±11cpm；阿霉素处理组，172±14cpm；平均值±标准误；P<0.001）。似乎阿霉素处理的动物中导致WBS降低的缺陷并非由于胶原成熟缺陷，而是由于通过新合成羟脯氨酸含量测量的瘢痕胶原积累减少以及通过EM测量确定的纤维直径减小。
