胎儿弯曲菌对马尿酸盐的水解作用及对三苯基四氮唑的耐受性
Hippurate hydrolysis by and triphenyltetrazolium tolerance of Campylobacter fetus.
作者信息
Luechtefeld N W, Wang W L
出版信息
J Clin Microbiol. 1982 Jan;15(1):137-40. doi: 10.1128/jcm.15.1.137-140.1982.
A rapid test of hippurate hydrolysis and a test of tolerance to triphenyltetrazolium chloride (TTC) were studied in 315 strains of Campylobacter fetus subsp. jejuni to determine their usefulness for biotyping this organism and for distinguishing it from C. fetus subsp. intestinalis. Of the 315 strains tested, 84% hydrolyzed hippurate and 97% were resistant to TTC. Ability to hydrolyze hippurate was seen in 99% of 155 human isolates, 75% of 60 avian isolates, 100% of 41 cattle and dog isolates, 84% of 31 zoo mammal isolates, and none of 28 hog isolates. Resistance to 400 micrograms of TTC per ml was seen in 97% of the human isolates, 95% of the avian isolates, and 100% of the mammalian isolates (other than human). In no case did any of the 315 isolates of C. fetus subsp. jejuni show both lack of ability to hydrolyze hippurate and sensitivity to TTC. In contrast, all 18 strains of C. fetus subsp. intestinalis failed to hydrolyze hippurate and were sensitive to TTC. These two tests may be useful to distinguish between C. fetus subsp. jejuni and subsp. intestinalis and also to biotype strains of C. fetus subsp. jejuni.
对315株空肠弯曲菌胎儿亚种进行了马尿酸盐水解快速试验和对氯化三苯基四氮唑（TTC）的耐受性试验，以确定它们在该菌生物分型以及将其与胎儿弯曲菌肠道亚种区分方面的实用性。在检测的315株菌株中，84%能水解马尿酸盐，97%对TTC耐药。在155株人类分离株中有99%、60株禽类分离株中有75%、41株牛和狗分离株中有100%、31株动物园哺乳动物分离株中有84%能水解马尿酸盐，而28株猪分离株中无一能水解。每毫升400微克TTC耐药性在97%的人类分离株、95%的禽类分离株以及100%的哺乳动物分离株（人类除外）中可见。315株空肠弯曲菌胎儿亚种分离株中无一既不能水解马尿酸盐又对TTC敏感。相比之下，所有18株胎儿弯曲菌肠道亚种均不能水解马尿酸盐且对TTC敏感。这两项试验可能有助于区分空肠弯曲菌胎儿亚种和肠道亚种，也有助于对空肠弯曲菌胎儿亚种菌株进行生物分型。
Zotero 插件