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正常人和糖尿病患者中糖化血红蛋白A1c的比色测定

The colorimetric determination of HbA1c in normal and diabetic subjects.

作者信息

Fischer R W, De Jong C, Voigt E, Berger W, Winterhalter K H

出版信息

Clin Lab Haematol. 1980;2(2):129-38. doi: 10.1111/j.1365-2257.1980.tb00816.x.

DOI:10.1111/j.1365-2257.1980.tb00816.x
PMID:7398202
Abstract

The parameters which influence the determination of the major glycosylated haemoglobin fraction (HbA1c) with the thiobarbituric acid (TBA) method are described. Conditions for an optimal determination method are given. The correlation with the fast haemoglobin determination by column chromatography is greater than 0.9. The influence of storage on blood, washed erythrocytes and haemolysate in respect to HbA1c values obtained with the method described, was also investigated. Normal and pathological values from 78 patients correlate well with the fasting blood sugar levels (r greater than 0.9).

摘要

描述了用硫代巴比妥酸(TBA)法测定主要糖化血红蛋白组分(HbA1c)时的影响参数。给出了最佳测定方法的条件。与柱色谱法快速测定血红蛋白的相关性大于0.9。还研究了血液、洗涤红细胞和溶血产物的储存对用所述方法获得的HbA1c值的影响。78例患者的正常和病理值与空腹血糖水平相关性良好(r大于0.9)。

相似文献

1
The colorimetric determination of HbA1c in normal and diabetic subjects.正常人和糖尿病患者中糖化血红蛋白A1c的比色测定
Clin Lab Haematol. 1980;2(2):129-38. doi: 10.1111/j.1365-2257.1980.tb00816.x.
2
A new colorimetric method for the estimation of glycosylated hemoglobin.一种用于估算糖化血红蛋白的新型比色法。
Clin Chim Acta. 1981 Feb 5;109(3):267-74. doi: 10.1016/0009-8981(81)90312-0.
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[Determination of glycosylated albumin and its clinical significance in diabetes mellitus].
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Comparison of a colorimetric assay for glycosylated hemoglobin with ion-exchange chromatography.糖化血红蛋白比色测定法与离子交换色谱法的比较。
Diabetes. 1979 Dec;28(12):1120-5. doi: 10.2337/diab.28.12.1120.
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The relationship between blood glycosylated haemoglobin and home capillary blood glucose levels in diabetics.糖尿病患者血液糖化血红蛋白与家庭毛细血管血糖水平之间的关系。
Diabetologia. 1980 Jul;19(1):31-4. doi: 10.1007/BF00258307.
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A semi-micro colorimetric method for estimating glycosylated haemoglobin.
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Determination of glycosylated haemoglobin in normal, newborn and diabetic Saudi Arabs.
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Glycosylated hemoglobin: colorimetric vs chromatographic measurement.
Diabetes Res. 1986 Sep;3(7):381-6.
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Rapid changes in chromatographically determined haemoglobin A1c induced by short-term changes in glucose concentration.由葡萄糖浓度短期变化引起的色谱法测定糖化血红蛋白A1c的快速变化。
Diabetologia. 1980 Aug;19(2):130-6. doi: 10.1007/BF00421859.
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Non-enzymatically glycosylated serum protein in diabetes mellitus: an index of short-term glycaemia.糖尿病中的非酶糖基化血清蛋白:短期血糖指标
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引用本文的文献

1
Measurement of glycosylated haemoglobin at high altitudes.高海拔地区糖化血红蛋白的测量。
Diabetologia. 1982 Jun;22(6):493. doi: 10.1007/BF00282599.
2
[Comparison of various methods for determining glycosylated hemoglobins].
Klin Wochenschr. 1984 Aug 16;62(16):787-92. doi: 10.1007/BF01721779.
3
Glycosylated haemoglobin: measurement and clinical use.糖化血红蛋白:测量与临床应用
J Clin Pathol. 1984 Aug;37(8):841-51. doi: 10.1136/jcp.37.8.841.
4
Bicentric evaluation of a teaching and treatment programme for type 1 (insulin-dependent) diabetic patients: improvement of metabolic control and other measures of diabetes care for up to 22 months.1型(胰岛素依赖型)糖尿病患者教学与治疗方案的双中心评估:长达22个月的代谢控制改善及糖尿病护理的其他指标
Diabetologia. 1983 Dec;25(6):470-6. doi: 10.1007/BF00284453.