Bailey E, Fenoughty M, Richardson L
J Chromatogr. 1977 Jan 21;131:347-55. doi: 10.1016/s0021-9673(00)80951-6.
An automated high-resolution gas chromatographic method has been developed for the determination of low levels of the antidepressant psychotropic drug Nomifensine in human plasma. The drug is extracted from alkalinized plasma with diethyl ether and then back-extracted into an acidic aqueous phase. After subsequent extraction into diethyl ether the drug is analysed by gas-liquid chromatography as its heptafluorobutyrate derivative using an OV-101 open-tubular glass capillary column with a nitrogen-specific detector. The propyl and butyl analogues of Nomifensine are used as internal standards, added to the plasma before extraction. The method is accurate, specific and precise, and capable of measuring plasma concentrations down to a level of 2 ng/ml. A preliminary study of the pharmacokinetics of the drug, together with steady-state level measurements in normal individuals receiving therapeutic dosages of Nomifensine, has been made.
已开发出一种自动化的高分辨率气相色谱法，用于测定人血浆中低水平的抗抑郁精神药物诺米芬辛。该药物从碱化血浆中用乙醚萃取，然后反萃取到酸性水相中。随后再萃取到乙醚中后，使用带有氮特异性检测器的OV-101开管玻璃毛细管柱，将该药物作为其七氟丁酸酯衍生物通过气液色谱法进行分析。诺米芬辛的丙基和丁基类似物用作内标，在萃取前添加到血浆中。该方法准确、特异且精密，能够测量低至2 ng/ml水平的血浆浓度。已对该药物的药代动力学进行了初步研究，并对接受治疗剂量诺米芬辛的正常个体进行了稳态水平测量。
