Peacock J H, Stephens T C
Br J Cancer. 1978 Dec;38(6):725-31. doi: 10.1038/bjc.1978.279.
The influence of anaesthetics on the in vivo response of B16 melanoma to melphalan was studied using an in vitro cell-survival assay. Three anaesthetics were used, Saffan (Althesin) Sagatal (Nembutal) and Hypnorm. When Saffan was administered to tumour-bearing animals before melphalan there was a significant increase in tumour-cell kill. This effect was not observed with Sagatal or Hypnorm. Maximum increase in tumour-cell kill was achieved when Saffan was administered about 1 h before melphalan, and was dependent on Saffan dose. Clonogenic tumour-cell repopulation after melphalan was rapid (TD - 1 day) and the rate was similar from 2 levels of cell kill. When Saffan was combined with melphalan the repopulation rate was the same as with melphalan alone, and the increased cell kill was reflected in increased growth delay. The in vitro response of B16 melanoma cells to melphalan was unaltered by pretreatment with, or simultaneous exposure to Saffan. The results suggest that the mechanism of the enhanced cell kill in vivo is probably due to an indirect systemic effect, rather than a direct effect on the tumour cells.
采用体外细胞存活试验研究了麻醉剂对B16黑色素瘤体内对美法仑反应的影响。使用了三种麻醉剂，舒泰（Althesin）、戊巴比妥（Nembutal）和氟哌利多/芬太尼合剂（Hypnorm）。当在给荷瘤动物使用美法仑之前给予舒泰时，肿瘤细胞杀伤显著增加。戊巴比妥或氟哌利多/芬太尼合剂未观察到这种效应。当在美法仑给药前约1小时给予舒泰时，肿瘤细胞杀伤增加最大，且依赖于舒泰剂量。美法仑后克隆源性肿瘤细胞再增殖迅速（TD - 1天），且在2个细胞杀伤水平下速率相似。当舒泰与美法仑联合使用时，再增殖速率与单独使用美法仑时相同，且细胞杀伤增加反映在生长延迟增加。用舒泰预处理或同时暴露于舒泰对B16黑色素瘤细胞对美法仑的体外反应无改变。结果表明，体内细胞杀伤增强的机制可能是由于间接的全身效应，而非对肿瘤细胞的直接效应。
