Masuno H, Yamasaki N, Okuda H
Cancer Res. 1981 Jan;41(1):284-8.
A lipolytic substance in the ascites fluid of mice with Sarcoma 180, called toxohormone-L, was purified and characterized. The lipolytic activity of toxohormone-L was measured in vitro using rat adipose tissue slices. Toxohormone-L, purified approximately 90-fold from the ammonium sulfate fraction, gave a single band on polyacrylamide gel electrophoresis. Its molecular weight was about 75,000, and its isoelectric point was 4.7. Toxohormone-L is heat labile and nondialyzable, but, on its digestion with trypsin, an active fragment that was heat stable and dialyzable was produced. Toxohormone-L is a protein and is also present in the ascites fluid of patients with hepatoma and Grawitz's tumor.
从患有肉瘤180的小鼠腹水中纯化并鉴定出一种名为毒素激素-L的脂解物质。使用大鼠脂肪组织切片在体外测量毒素激素-L的脂解活性。从硫酸铵级分中纯化约90倍的毒素激素-L在聚丙烯酰胺凝胶电泳上呈现单一谱带。其分子量约为75,000，等电点为4.7。毒素激素-L对热不稳定且不可透析，但在用胰蛋白酶消化时，会产生一种对热稳定且可透析的活性片段。毒素激素-L是一种蛋白质，也存在于肝癌和肾上腺皮质瘤患者的腹水中。
