Gorczynski R M, MacRae S
Br J Cancer. 1981 Jan;43(1):19-31. doi: 10.1038/bjc.1981.4.
An assay system is described in which effector cells added along with suitable target cells inhibit, in a quantitative fashion, the subsequent uptake of 3H-thymidine by those target cells. Effector cells active in this assay, using embryonic fibroblast cells as targets, develop spontaneously in cultures of mouse lymphoid cells, but are apparently different from those described earlier by investigators of activity in cytotoxic assays. Further evidence is presented to show the development of spleen-derived effector cells with cytostatic activity (for embryonic fibroblast target cells) in mice during the course of normal pregnancy, or growth of spontaneously appearing mammary adenocarcinomas. Indeed, such effector cells can also be found within the growing solid mass itself. Different populations of tumour cells isolated from a solid tumour apparently differ in their susceptibility to growth inhibition by tumour-bearer-derived cytostatic effector cells, a phenomenon which may be related to metastatic spread of tumour cells.
本文描述了一种检测系统，在该系统中，将效应细胞与合适的靶细胞一起添加，可定量抑制这些靶细胞随后对³H-胸腺嘧啶核苷的摄取。以胚胎成纤维细胞为靶细胞，在此检测中具有活性的效应细胞在小鼠淋巴细胞培养物中自发形成，但显然不同于早期细胞毒性检测研究人员所描述的那些细胞。进一步的证据表明，在正常妊娠过程中，小鼠体内会产生具有细胞抑制活性（针对胚胎成纤维细胞靶细胞）的脾源性效应细胞，或者自发出现的乳腺腺癌会生长。实际上，在生长的实体瘤内部也能发现此类效应细胞。从实体瘤中分离出的不同肿瘤细胞群体，对荷瘤动物来源的细胞抑制效应细胞的生长抑制敏感性明显不同，这一现象可能与肿瘤细胞的转移扩散有关。
