Parant M, Riveau G, Parant F, Dinarello C A, Wolff S M, Chedid L
J Infect Dis. 1980 Nov;142(5):708-15. doi: 10.1093/infdis/142.5.708.
The pyrogenicity in the rabbit and the ability to stimulate nonspecific resistance to bacterial infection in the mouse of muramyl dipeptide (MDP), a synthetic immunoadjuvant, are known to be enhanced when the glycopeptide has been conjugated to a carrier. The effects of indomethacin on fever induced by MDP or its conjugated derivative were studied. Indomethacin reduced febrile responses to MDP or its derivative, although it did not decrease production of endogenous pyrogen in vivo or in vitro. When incubated with rabbit peritoneal cells, indomethacin suppressed the elevation in prostaglandin levels usually induced by MDP. When administered to mice, indomethacin alone stimulated resistance to bacterial infection and, under appropriate conditions, had a strong synergistic effect when combined with free or conjugated MDP. The results demonstrate that neither pyrogenicity nor increased prostaglandin levels are prerequisites for immunopotentiation by synthetic glycopeptides.
已知当糖肽与载体结合时，合成免疫佐剂胞壁酰二肽（MDP）在兔体内的致热原性以及在小鼠体内刺激对细菌感染的非特异性抵抗力的能力会增强。研究了吲哚美辛对MDP或其共轭衍生物诱导的发热的影响。吲哚美辛降低了对MDP或其衍生物的发热反应，尽管它没有降低体内或体外内源性致热原的产生。当与兔腹膜细胞一起孵育时，吲哚美辛抑制了通常由MDP诱导的前列腺素水平的升高。当给小鼠给药时，单独的吲哚美辛刺激对细菌感染的抵抗力，并且在适当的条件下，与游离或共轭MDP联合使用时具有很强的协同作用。结果表明，合成糖肽免疫增强作用既不需要致热原性也不需要升高的前列腺素水平。
