[用特异性核酸内切酶R. SaLI、R. EcoRI和R. BamHI切割人1型腺病毒DNA]
[Fragmentation of human adenovirus type 1 DNA by the specific endonucleases R. SaLI, R. EcoRI and R. BamHI].
作者信息
Zavizion B A, Chaplygina N M, Naroditskiĭ B S, Tikhonenko T I, Berenchi D
出版信息
Vopr Virusol. 1978 Nov-Dec(6):650-5.
The restricting endonuclease R. EcoRI hydrolyses human adenovirus type I (Adl) DNA with formation of three fragments with molecular weights of 17.5 x 10(6), 3.7 x 10(6), and 1.55 x 10(6) dalton. Under the effect of R. SaLI restrictase DNA Adl disintegrates into 5 fragments with molecular weights: 8.0 x 10(6), 6.0 x 10(6), 4.6 x 10(6), 4.0 x 10(6), and 0.2 x 10(6) dalton. Treatment of DNA with R. BamHI produces 4 fragments with molecular weights 10.0 x 10(6), 6.8 x 10(6), 4.0 x 10(6), and 2.2 x 10(6) dalton. Fragments of R. SaLI and R. BamHI form sequences BECAD and ACBD, respectively.
限制性内切酶R. EcoRI可水解I型人腺病毒（Adl）DNA，形成三个分子量分别为17.5×10⁶、3.7×10⁶和1.55×10⁶道尔顿的片段。在R. SaLI限制性内切酶的作用下，Adl DNA分解为5个片段，分子量分别为：8.0×10⁶、6.0×10⁶、4.6×10⁶、4.0×10⁶和0.2×10⁶道尔顿。用R. BamHI处理DNA会产生4个片段，分子量分别为10.0×10⁶、6.8×10⁶、4.0×10⁶和2.2×10⁶道尔顿。R. SaLI和R. BamHI的片段分别形成序列BECAD和ACBD。
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