Herrmann J E, Blacklow N R, Matsui S M, Lewis T L, Estes M K, Ball J M, Brinker J P
Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester 01655, USA.
J Clin Microbiol. 1995 Sep;33(9):2511-3. doi: 10.1128/jcm.33.9.2511-2513.1995.
Monoclonal antibodies against the prototype 8FIIa strain of Norwalk virus were prepared and applied to an enzyme immunoassay (EIA) for detecting Norwalk virus in stool specimens. The monoclonal antibodies immunoprecipitated a 58-kDa protein which had been produced by in vitro transcription-translation of Norwalk virus cloned cDNA, and they reacted by EIA with recombinant Norwalk virus capsid protein at a sensitivity level of 1 ng/ml. The EIA detected virus in all tested samples from 15 different Norwalk virus-infected volunteers. No cross-reactions were seen in stools containing other caliciviruses or in stools containing rotaviruses, astroviruses, or enteric adenoviruses.
制备了针对诺沃克病毒原型8FIIa株的单克隆抗体，并将其应用于酶免疫测定（EIA），以检测粪便标本中的诺沃克病毒。这些单克隆抗体免疫沉淀了一种58 kDa的蛋白质，该蛋白质是通过诺沃克病毒克隆cDNA的体外转录-翻译产生的，并且它们通过EIA与重组诺沃克病毒衣壳蛋白反应，灵敏度为1 ng/ml。EIA在来自15名不同诺沃克病毒感染志愿者的所有测试样本中检测到了病毒。在含有其他杯状病毒的粪便中或含有轮状病毒、星状病毒或肠道腺病毒的粪便中未观察到交叉反应。
