用酶免疫测定法检测粪便中的诺如病毒。

Detection of Norwalk virus in stools by enzyme immunoassay.

作者信息

Herrmann J E, Nowak N A, Blacklow N R

出版信息

J Med Virol. 1985 Oct;17(2):127-33. doi: 10.1002/jmv.1890170205.

Abstract

The development of a solid-phase microtiter enzyme immunoassay (EIA) for detection of Norwalk virus antigen in stool samples is described. The EIA was compared with a previously developed radioimmunoassay (RIA) for detection of Norwalk virus antigen in stools obtained from 30 volunteers who received Norwalk virus. The EIA detected viral antigen in stools from 17 of the volunteers and the RIA detected viral antigen in 15. Seroconversion was a more sensitive indicator of infection in some patients. However, two samples from volunteers who were clinically ill but did not show seroconversion to Norwalk virus were positive for Norwalk virus antigen by both immunoassays. This indicates that antigen detection may be important for use in epidemiological studies. Neither of the immunoassays gave positive reactions for stools known to contain enteric adenovirus, rotavirus, or Hawaii virus, or in stools from patients with acute diarrhea of unknown cause. The stability of the EIA reagents and ease of use should provide a means for more extensive testing for Norwalk virus in outbreaks of gastroenteritis.

摘要

本文描述了一种用于检测粪便样本中诺沃克病毒抗原的固相微量滴定酶免疫测定法（EIA）。将该EIA与先前开发的用于检测从30名接种诺沃克病毒的志愿者粪便中诺沃克病毒抗原的放射免疫测定法（RIA）进行了比较。EIA在17名志愿者的粪便中检测到病毒抗原，RIA在15名志愿者的粪便中检测到病毒抗原。血清转化在某些患者中是更敏感的感染指标。然而，两名临床患病但未显示对诺沃克病毒血清转化的志愿者的样本，两种免疫测定法均检测出诺沃克病毒抗原呈阳性。这表明抗原检测在流行病学研究中可能很重要。两种免疫测定法对已知含有肠道腺病毒、轮状病毒或夏威夷病毒的粪便，或对病因不明的急性腹泻患者的粪便均未产生阳性反应。EIA试剂的稳定性和易用性应为在胃肠炎暴发中更广泛地检测诺沃克病毒提供一种方法。