Musser S M, Eppley R M, Mazzola E P, Hadden C E, Shockcor J P, Crouch R C, Martin G E
Center for Food Safety and Applied Nutrition, Food and Drug Administration, Washington, DC 20204, USA.
J Nat Prod. 1995 Sep;58(9):1392-7. doi: 10.1021/np50123a009.
A method is presented for the separation and identification of a new N-acetyl keto derivative of fumonisin B1 (FB1) produced in solid corn culture. Cultures of Fusarium proliferatum (M-1597) were purified using preparative hplc, and the new fumonisin was detected by negative-ion esms. Structures were confirmed by 1H- and 13C-nmr spectroscopy. The new fumonisin differs from FB1 in that the tricarballylic acid functionality at the C-15 position of the eicosane backbone is replaced by a ketone and the amino group is acetylated. Direct analysis of the culture material by negative-ion electrospray lc/ms confirmed that the new fumonisin is produced naturally by the fungus.
本文介绍了一种用于分离和鉴定在固体玉米培养物中产生的伏马毒素B1（FB1）新的N-乙酰基酮衍生物的方法。使用制备型高效液相色谱法对层出镰刀菌（M-1597）培养物进行纯化，并通过负离子电喷雾质谱法检测新的伏马毒素。通过1H-和13C-核磁共振光谱确定结构。新的伏马毒素与FB1的不同之处在于，二十烷主链C-15位的三羧酸官能团被酮取代，且氨基被乙酰化。通过负离子电喷雾液相色谱/质谱对培养物材料进行直接分析，证实该新伏马毒素是由真菌天然产生的。
