Ausma J, Cleutjens J, Thoné F, Flameng W, Ramaekers F, Borgers M
Department of Molecular Cell Biology and Genetics, Cardiovascular Research Institute Maastricht, University of Limburg, The Netherlands.
Mol Cell Biochem. 1995;147(1-2):35-42. doi: 10.1007/BF00944781.
Chronic left ventricular dysfunctional but viable myocardium of patients with chronic hibernation is characterized by structural changes, which consist of depletion of contractile elements, accumulation of glycogen, nuclear chromatin dispersion, depletion of sarcoplasmic reticulum and mitochondrial shape changes. These alterations are not reminiscent of degeneration but are interpreted as de-differentiation of the cardiomyocytes. The above mentioned changes are accompanied by a marked increase in the interstitial space. The present study describes qualitative and quantitative changes in the cellular and non-cellular compartments of the interstitial space. In chronic hibernating myocardial segments the increased extracellular matrix is filled with large amounts of type I collagen, type III collagen and fibronectin. An increase in the number of vimentin-positive cells (endothelial cells and fibroblasts) compared with normal myocardium is seen throughout the extracellular matrix. The increase in interstitial tissue is considered as one of the main determinants responsible for the lack of immediate recovery of contractile function after restoration of the blood flow to the affected myocardial segments of patients with chronic left ventricular dysfunction.
慢性冬眠患者的慢性左心室功能不全但存活的心肌以结构变化为特征，这些变化包括收缩元件的耗竭、糖原的积累、核染色质分散、肌浆网的耗竭以及线粒体形态改变。这些改变并非退行性变，而是被解释为心肌细胞的去分化。上述变化伴随着间质空间的显著增加。本研究描述了间质空间细胞和非细胞成分的定性和定量变化。在慢性冬眠心肌节段中，增加的细胞外基质充满了大量的I型胶原、III型胶原和纤连蛋白。与正常心肌相比，在整个细胞外基质中可见波形蛋白阳性细胞（内皮细胞和成纤维细胞）数量增加。间质组织的增加被认为是导致慢性左心室功能不全患者受影响心肌节段恢复血流后收缩功能不能立即恢复的主要决定因素之一。
