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串联重复序列AGGGTAGGGT在裂殖酵母中是一个近端激活序列,可激活由序列TGTGACTG介导的基础转录。

The tandem repeat AGGGTAGGGT is, in the fission yeast, a proximal activation sequence and activates basal transcription mediated by the sequence TGTGACTG.

作者信息

Witt I, Kwart M, Gross T, Käufer N F

机构信息

Institut für Biochemie und Molekularbiologie, Freie Universität Berlin, Germany.

出版信息

Nucleic Acids Res. 1995 Nov 11;23(21):4296-302. doi: 10.1093/nar/23.21.4296.

Abstract

Ribosomal protein (rp) genes in the fission yeast Schizosaccharomyces pombe display two highly conserved sequence elements in the promoter region. The molecular dissection of these promoters revealed that basal transcription is not based on a TATA element. The sequence which promotes basal transcription is the conserved sequence CAGTCACA or the inverted form TGTGACTG, called the homol D box. Upstream of the homol D box a tandem repeat AGGGTAGGGT or the inverted form ACCCTACCCT appears in some promoters, called homol E. This element functions in the proximal arrangement with homol D as an activation sequence. A compilation of homol D and homol E sequences identified in other S.pombe promoters revealed that several putative polymerase II and polymerase III promoters display a homol D box or the homol E/homol D arrangement.

摘要

裂殖酵母粟酒裂殖酵母中的核糖体蛋白(rp)基因在启动子区域显示出两个高度保守的序列元件。对这些启动子的分子剖析表明,基础转录并非基于TATA元件。促进基础转录的序列是保守序列CAGTCACA或其反向形式TGTGACTG,称为同源D框。在同源D框的上游,一些启动子中会出现串联重复AGGGTAGGGT或其反向形式ACCCTACCCT,称为同源E。该元件与同源D近端排列时作为激活序列发挥作用。对在其他粟酒裂殖酵母启动子中鉴定出的同源D和同源E序列的汇编表明,几个推定的聚合酶II和聚合酶III启动子显示出同源D框或同源E/同源D排列。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/325e/307383/b8760263f98f/nar00021-0081-a.jpg

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