Bensimon A, Simon A, Chiffaudel A, Croquette V, Heslot F, Bensimon D
Institut Pasteur, Paris, France.
Science. 1994 Sep 30;265(5181):2096-8. doi: 10.1126/science.7522347.
In a process called "molecular combining," DNA molecules attached at one end to a solid surface were extended and aligned by a receding air-water interface and left to dry on the surface. Molecular combing was observed to extend the length of the bacteriophage lambda DNA molecule to 21.5 +/- 0.5 micrometers (unextended length, 16.2 micrometers). With the combing process, it was possible to (i) extend a chromosomal Escherichia coli DNA fragment (10(6) base pairs) and (ii) detect a minute quantity of DNA (10(3) molecules). These results open the way for a faster physical mapping of the genome and for the detection of small quantities of target DNA from a population of molecules.
在一个被称为“分子梳理”的过程中，一端附着在固体表面的DNA分子通过后退的气-水界面被拉伸并排列整齐，然后留在表面干燥。观察到分子梳理可将噬菌体λ DNA分子的长度延长至21.5±0.5微米（未拉伸长度为16.2微米）。通过梳理过程，可以（i）拉伸大肠杆菌染色体DNA片段（10⁶个碱基对），以及（ii）检测微量DNA（10³个分子）。这些结果为更快地进行基因组物理图谱绘制以及从分子群体中检测少量目标DNA开辟了道路。
