Sandhu S S, de Serres F J, Gopalan H N, Grant W F, Velemínský J, Becking G C
Health Effects Research Laboratory, Environmental Protection Agency, Research Triangle Park, NC 27711.
Mutat Res. 1994 Oct 16;310(2):169-73. doi: 10.1016/0027-5107(94)90111-2.
Under the sponsorship of the International Programme on Chemical Safety (IPCS), 17 laboratories from diverse regions of the world participated in evaluating the utility of four plant bioassays for detecting genetic hazards of environmental chemicals. The bioassays included in this collaborative study were: Arabidopsis thaliana embryo and chlorophyll assay and Tradescantia stamen hair assay, Tradescantia paludosa micronucleus assay and Vicia faba root tip assay. Four to six laboratories participated in the performance of each of the bioassays. All laboratories participating in a particular bioassay were supplied with uniform plant material as well as standardized protocol. Five direct acting water soluble test chemicals, i.e. maleic hydrazide, methyl nitrosourea, ethyl methanesulfonate, sodium azide and azidoglycerol, were selected for this study. The study was designed to be completed in three phases. Ethyl methanesulfonate was used as a positive control and has already been reported earlier (Sandhu et al., 1991). The data from the remaining four chemicals used for the evaluation of four plant test systems in the first phase of the collaborative study are reported in this issue.
在国际化学品安全规划署（IPCS）的赞助下，来自世界不同地区的17个实验室参与了评估四种植物生物测定法检测环境化学品遗传危害的效用。这项合作研究中包括的生物测定法有：拟南芥胚胎和叶绿素测定法、紫露草雄蕊毛测定法、湿地紫露草微核测定法和蚕豆根尖测定法。每种生物测定法由四至六个实验室参与实施。参与特定生物测定法的所有实验室都获得了统一的植物材料以及标准化方案。本研究选择了五种直接作用的水溶性测试化学品，即马来酰肼、甲基亚硝基脲、甲磺酸乙酯、叠氮化钠和叠氮甘油。该研究计划分三个阶段完成。甲磺酸乙酯用作阳性对照，此前已有报道（Sandhu等人，1991年）。本期报告了合作研究第一阶段用于评估四种植物测试系统的其余四种化学品的数据。
