拟南芥致突变性测定

Arabidopsis assay for mutagenicity.

作者信息

Gichner T, Badayev S A, Demchenko S I, Relichová J, Sandhu S S, Usmanov P D, Usmanova O, Velemínský J

机构信息

Institute of Experimental Botony, Prague, Czech Republic.

出版信息

Mutat Res. 1994 Oct 16;310(2):249-56. doi: 10.1016/0027-5107(94)90117-1.

DOI:10.1016/0027-5107(94)90117-1

PMID:7523895

Abstract

Four laboratories, two in the Czech Republic (Brno and Prague) and two in the CIS (Moscow and Duschanbe), participated in the International Programme on Chemical Safety's (IPCS) collaborative study to evaluate the utility of the most commonly used plant test systems, including the Arabidopsis thaliana assay, for assessing the mutagenic potential of environmental agents. Out of the five compounds evaluated in the Arabidopsis assay, three compounds, i.e., ethyl methanesulfonate, N-methyl-N-nitrosourea, and azidoglycerol, were reported to be mutagenic by all four participating laboratories. Sodium azide (NaN3) demonstrated a negative response in all four laboratories, whereas maleic hydrazide was reported to be weakly mutagenic by one laboratory and nonmutagenic by the other three laboratories.

摘要

四个实验室参与了国际化学品安全规划署（IPCS）的合作研究，其中两个位于捷克共和国（布尔诺和布拉格），两个位于独联体（莫斯科和杜尚别），目的是评估包括拟南芥试验在内的最常用植物测试系统在评估环境因子致突变潜力方面的效用。在拟南芥试验中评估的五种化合物中，四种参与实验室均报告称三种化合物，即甲磺酸乙酯、N-甲基-N-亚硝基脲和叠氮甘油具有致突变性。叠氮化钠（NaN3）在所有四个实验室中均显示出阴性反应，而马来酰肼在一个实验室中被报告为弱致突变性，在其他三个实验室中被报告为无致突变性。