Kanaya N, Gill B S, Grover I S, Murin A, Osiecka R, Sandhu S S, Andersson H C
Department of Biology, Keio University, Yokohama, Japan.
Mutat Res. 1994 Oct 16;310(2):231-47. doi: 10.1016/0027-5107(94)90116-3.
A collaborative study involving laboratories in six countries was initiated under the sponsorship of the International Programme on Chemical Safety (IPCS) to determine the sensitivity, efficiency and reliability of the Vicia faba root tip meristem chromosomal aberration assay using a standardized protocol. The six laboratories that participated in this study were located in the Slovak Republic, India, Japan, Poland, Sweden and the USA. All laboratories adhered to a standardized protocol for the Vicia faba chromosomal aberration assay. Four coded chemicals, azidoglycerol (AG), N-methyl-N-nitrosourea (MNU), sodium azide (NaN3) and maleic hydrazide (MH) were tested with the Vicia faba chromosomal aberration assay. Of the four chemicals, three (MH, AG and MNU) were found to be clastogenic and gave a concentration related response. However, the results of NaN3 were equivocal which might be explained by the stability of NaN3. The conclusions from this study suggest that the Vicia faba chromosomal aberration bioassay is an efficient and reliable short-term bioassay for the rapid screening of chemicals for clastogenicity.
在国际化学品安全计划（IPCS）的赞助下，发起了一项涉及六个国家实验室的合作研究，以使用标准化方案确定蚕豆根尖分生组织染色体畸变试验的敏感性、效率和可靠性。参与该研究的六个实验室分别位于斯洛伐克共和国、印度、日本、波兰、瑞典和美国。所有实验室都遵循蚕豆染色体畸变试验的标准化方案。用蚕豆染色体畸变试验对四种编码化学品叠氮甘油（AG）、N-甲基-N-亚硝基脲（MNU）、叠氮化钠（NaN3）和顺丁烯二酸酰肼（MH）进行了测试。在这四种化学品中，有三种（MH、AG和MNU）被发现具有致断裂性，并呈现出浓度相关反应。然而，NaN3的结果不明确，这可能是由NaN3的稳定性所解释的。该研究的结论表明，蚕豆染色体畸变生物测定法是一种高效且可靠的短期生物测定法，可用于快速筛选化学品的致断裂性。
