Ma T H, Cabrera G L, Chen R, Gill B S, Sandhu S S, Vandenberg A L, Salamone M F
Department of Biological Sciences, Western Illinois University, Macomb 61455.
Mutat Res. 1994 Oct 16;310(2):221-30. doi: 10.1016/0027-5107(94)90115-5.
Four coded chemicals, azidoglycerol (AG), N-methyl-N-nitrosourea (MNU), sodium azide (NaN3), and maleic hydrazide (MH), were tested with the Tradescantia micronucleus (Trad-MCN) bioassay by five independent laboratories from five different countries. The purpose of this international collaborative study was to evaluate four plant bioassays, of which the Trad-MCN assay was one, for their sensitivity, efficiency and reliability. The study was carried out under the sponsorship of the International Programme on Chemical Safety. All laboratories adhered to a standard Trad-MCN protocol which suggested that three replicate tests be conducted with each chemical. The results reported by all laboratories, although not equal, showed good agreement among the laboratories. In fact, all five laboratories obtained positive results with MH and MNU, while four of the five laboratories achieved positive results with NaN3. AG was tested in only three laboratories. Two reported negative results, while one reported positive results but only at a single high dose. The data from this study suggest that under normal conditions, the Trad-MCN bioassay is an efficient and reliable short-term bioassay for clastogens. It is suitable for the rapid screening of chemicals, and also is specially qualified for in situ monitoring of ambient pollutants.
来自五个不同国家的五个独立实验室，使用紫露草微核（Trad-MCN）生物测定法对四种编码化学品——叠氮甘油（AG）、N-甲基-N-亚硝基脲（MNU）、叠氮化钠（NaN₃）和顺丁烯二酸肼（MH）进行了测试。这项国际合作研究的目的是评估四种植物生物测定法（其中包括Trad-MCN测定法）的灵敏度、效率和可靠性。该研究是在国际化学品安全计划的赞助下开展的。所有实验室均遵循标准的Trad-MCN方案，该方案建议对每种化学品进行三次重复测试。所有实验室报告的结果虽不完全相同，但各实验室之间显示出良好的一致性。事实上，所有五个实验室对MH和MNU均得到了阳性结果，而五个实验室中有四个对NaN₃得到了阳性结果。仅在三个实验室对AG进行了测试。两个实验室报告了阴性结果，而一个实验室报告了阳性结果，但仅在单一高剂量下。这项研究的数据表明，在正常条件下，Trad-MCN生物测定法是一种用于检测致断裂剂的高效且可靠的短期生物测定法。它适用于化学品的快速筛选，也特别适合对环境污染物进行现场监测。
