Ma T H, Cabrera G L, Cebulska-Wasilewska A, Chen R, Loarca F, Vandenberg A L, Salamone M F
Department of Biological Sciences, Western Illinois University, Macomb 61455.
Mutat Res. 1994 Oct 16;310(2):211-20. doi: 10.1016/0027-5107(94)90114-7.
The Tradescantia stamen hair mutation (Trad-SH) assay (clone 4430) was evaluated for its efficiency and reliability as a screen for mutagens in an IPCS collaborative study on plant systems. Four coded chemicals, i.e. azidoglycerol (AG, 3-azido-1,2-propanediol), N-methyl-N-nitrosourea (MNU), sodium azide (NaN3) and maleic hydrazide (MH) were distributed by the Radian Corporation to the five laboratories in five different countries for testing mutagenicity. Pink mutations were scored between the 7th and 14th day according to a standard protocol. Test results from the five individual laboratories were analyzed and compared after decoding. One out of the two laboratories that conducted tests on AG demonstrated that AG is a mutagen with genetically effective doses ranging from 50 to 100 micrograms/ml. MH yielded positive responses in all laboratories but no linear dose-response pattern was observed. The effective dose range for MH was between 1 and 45 micrograms/ml. The mutagenicity of MNU was reported by five laboratories in the dose range between 10 and 80 micrograms/ml. NaN3, which exhibited a relatively high degree of toxicity, elicited a positive mutagenic response in three of the five laboratories in which it was tested. As with MNU the effective dose for NaN3 ranged between 3 and 80 micrograms/ml. The results from the current study substantiate the Trad-SH assay as a reliable system for screening chemicals for their potential mutagenic effects. Although the study was carried out exclusively under laboratory conditions, a survey of the current literature would indicate that the Trad-SH assay could be an effective in situ monitor of gaseous, liquid, and radioactive pollutants as well.
在国际化学品安全规划署（IPCS）关于植物系统的一项合作研究中，对紫露草雄蕊毛突变试验（Trad-SH，克隆4430）作为诱变剂筛选方法的效率和可靠性进行了评估。Radian公司将四种编码化学品，即叠氮甘油（AG，3-叠氮基-1,2-丙二醇）、N-甲基-N-亚硝基脲（MNU）、叠氮化钠（NaN₃）和顺丁烯二酸酰肼（MH）分发给五个不同国家的五个实验室，以测试其致突变性。根据标准方案，在第7天至第14天对粉色突变进行评分。解码后，对五个独立实验室的测试结果进行了分析和比较。对AG进行测试的两个实验室中有一个表明，AG是一种诱变剂，其遗传有效剂量范围为50至100微克/毫升。MH在所有实验室中均产生了阳性反应，但未观察到线性剂量反应模式。MH的有效剂量范围为1至45微克/毫升。五个实验室报告了MNU在10至80微克/毫升剂量范围内的致突变性。NaN₃表现出相对较高的毒性，在对其进行测试的五个实验室中有三个产生了阳性诱变反应。与MNU一样，NaN₃的有效剂量范围为3至80微克/毫升。本研究结果证实了Trad-SH试验是一种可靠的系统，可用于筛选化学品的潜在诱变作用。尽管该研究仅在实验室条件下进行，但对当前文献的调查表明，Trad-SH试验也可能是气态、液态和放射性污染物的有效现场监测方法。
