Crossed immunoelectrofocusing of cerebrospinal fluid immunoglobulins.

The combination of the high separation capacity of isoelectric focusing and the specificity of immunoelectrophoresis gives a valuable tool for protein studies. This technic can be applied to immunoglobulin examinations by using KOCN-treated antibodies isoelectric at pH 5 and performing the immunoelectrophoretic procedure at this pH. CSF and serum samples of patients exhibiting CSF immunoglobulin abnormalities and blood-CSF barrier damages were examined by this method.