• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

脑脊液免疫球蛋白的交叉免疫电聚焦

Crossed immunoelectrofocusing of cerebrospinal fluid immunoglobulins.

作者信息

Sidén A

出版信息

J Neurol. 1977 Dec 13;217(2):103-9. doi: 10.1007/BF00312923.

DOI:10.1007/BF00312923
PMID:75256
Abstract

The combination of the high separation capacity of isoelectric focusing and the specificity of immunoelectrophoresis gives a valuable tool for protein studies. This technic can be applied to immunoglobulin examinations by using KOCN-treated antibodies isoelectric at pH 5 and performing the immunoelectrophoretic procedure at this pH. CSF and serum samples of patients exhibiting CSF immunoglobulin abnormalities and blood-CSF barrier damages were examined by this method.

摘要

等电聚焦的高分离能力与免疫电泳的特异性相结合，为蛋白质研究提供了一种有价值的工具。通过使用在pH 5时等电的经氰酸钾处理的抗体，并在此pH下进行免疫电泳程序，该技术可应用于免疫球蛋白检测。用这种方法检测了表现出脑脊液免疫球蛋白异常和血脑屏障损伤的患者的脑脊液和血清样本。

相似文献

1
Crossed immunoelectrofocusing of cerebrospinal fluid immunoglobulins.脑脊液免疫球蛋白的交叉免疫电聚焦
J Neurol. 1977 Dec 13;217(2):103-9. doi: 10.1007/BF00312923.
2
Crossed immunoelectrofocusing for identification of normal and abnormal cerebrospinal fluid proteins. A preliminary report.交叉免疫电聚焦用于鉴定正常和异常脑脊液蛋白质。初步报告。
J Neurol Sci. 1977 Jul;32(3):331-6. doi: 10.1016/0022-510x(77)90016-8.
3
The normal cerebrospinal fluid proteins identified by means of thin-layer isoelectric focusing and crossed immunoelectrofocusing.通过薄层等电聚焦和交叉免疫电聚焦鉴定的正常脑脊液蛋白质。
J Neurol Sci. 1978 Apr;36(2):273-88. doi: 10.1016/0022-510x(78)90088-6.
4
Isoelectric focusing and crossed immunoelectrofocusing of cerebrospinal fluid proteins in neurological disorders.神经系统疾病中脑脊液蛋白质的等电聚焦和交叉免疫电聚焦
Acta Neurol (Napoli). 1978 Dec;33(6):501-17.
5
Isoelectric focusing and crossed immunoelectrofocusing of CSF immunoglobulins in MS.多发性硬化症患者脑脊液免疫球蛋白的等电聚焦和交叉免疫电聚焦
J Neurol. 1979 Jul 11;221(1):39-51. doi: 10.1007/BF00313168.
6
Direct immunofixation after isoelectric focusing. An improved method for identification of cerebrospinal fluid and serum proteins.等电聚焦后的直接免疫固定。一种鉴定脑脊液和血清蛋白的改进方法。
J Neurol Sci. 1979 Jul;42(2):275-81. doi: 10.1016/0022-510x(79)90059-5.
7
Localization of the immunoglobulins G, A and M, beta-trace protein and gamma-trace protein on isoelectric focusing of serum and cerebrospinal fluid by immunofixation.通过免疫固定法对血清和脑脊液进行等电聚焦，以定位免疫球蛋白G、A和M、β-微量蛋白和γ-微量蛋白。
Acta Neurol Scand. 1978 Sep;58(3):141-7. doi: 10.1111/j.1600-0404.1978.tb02873.x.
8
[Bidimensional electrophoresis in the study of cerebrospinal fluid protein (author's transl)].二维电泳在脑脊液蛋白质研究中的应用（作者译）
Pathol Biol (Paris). 1976 Nov;24(9):593-9.
9
[Electrophoretic and immunoelectrophoretic studies in multiple sclerosis].[多发性硬化症的电泳和免疫电泳研究]
Neurol Psihiatr Neurochir. 1972 Mar-Apr;17(2):169-78.
10
[Changes in cerebrospinal fluid immunoglobulins during multiple sclerosis].[多发性硬化症期间脑脊液免疫球蛋白的变化]
Riv Patol Nerv Ment. 1969 Apr;90(2):99-112.

引用本文的文献

1
Analysis of immunoglobulin G in multiple sclerosis brain: quantitative and isoelectric focusing studies.多发性硬化症脑部免疫球蛋白G分析：定量及等电聚焦研究
Clin Exp Immunol. 1982 Apr;48(1):102-10.
2
Intrathecal IgG synthesis in multiple sclerosis: comparison between isoelectric focusing and quantitative estimation of cerebrospinal fluid IgG.多发性硬化症中鞘内IgG合成：等电聚焦与脑脊液IgG定量评估的比较
J Neurol. 1981;224(3):159-69. doi: 10.1007/BF00313278.
3
Abnormal CSF immunoglobulin components detected by isoelectric focusing.通过等电聚焦检测到脑脊液免疫球蛋白成分异常。

本文引用的文献

1
Isoelectric focusing of human urinary proteins in polyacrylamide gel.人尿蛋白在聚丙烯酰胺凝胶中的等电聚焦
Clin Chim Acta. 1970 Jul;29(1):101-5. doi: 10.1016/0009-8981(70)90229-9.
2
Recent developments in chemical modification and sequential degradation of proteins.蛋白质化学修饰与序列降解的最新进展。
Adv Protein Chem. 1970;24:261-308. doi: 10.1016/s0065-3233(08)60243-0.
3
Crossed immunoelectrophoresis and electroimmunoassay of IgM.IgM的交叉免疫电泳和免疫电泳分析
J Neurol. 1980;224(2):133-44. doi: 10.1007/BF00313351.
4
Isoelectric focusing and quantitative estimation of cerebrospinal fluid and serum IgG in idiopathic polyneuropathy.特发性多神经病中脑脊液和血清IgG的等电聚焦及定量测定
J Neurol. 1980;223(1):1-12. doi: 10.1007/BF00313135.
5
Isoelectric focusing and crossed immunoelectrofocusing of CSF immunoglobulins in MS.多发性硬化症患者脑脊液免疫球蛋白的等电聚焦和交叉免疫电聚焦
J Neurol. 1979 Jul 11;221(1):39-51. doi: 10.1007/BF00313168.
J Immunol. 1974 Apr;112(4):1420-5.
4
Carbamylated antibodies used for quantitation of human IgG. A routine method.
Scand J Immunol Suppl. 1973;1:145-8. doi: 10.1111/j.1365-3083.1973.tb03795.x.
5
Crossed immunoelectrophoresis and electroimmunoassay of IgG.IgG的交叉免疫电泳和免疫电泳分析
J Immunol. 1974 Jul;113(1):343-7.
6
Gel isoelectric focusing of cerebrospinal fluid proteins: a potential diagnostic tool.脑脊液蛋白质的凝胶等电聚焦：一种潜在的诊断工具。
Z Klin Chem Klin Biochem. 1971 Jul;9(4):334-6. doi: 10.1515/cclm.1971.9.4.334.
7
Isoelectric focusing of CSF proteins in neurological diseases.神经系统疾病中脑脊液蛋白质的等电聚焦
J Neurol Sci. 1974 Oct;23(2):199-213. doi: 10.1016/0022-510x(74)90223-8.
8
Thin layer isoelectric focusing of cerebrospinal fluid proteins. A preliminary report with special reference to the diagnostic significance in multiple sclerosis.脑脊液蛋白质的薄层等电聚焦。特别参考其在多发性硬化症诊断意义的初步报告。
Acta Neurol Scand Suppl. 1972;51:379-80.
9
[Comparative results of agar electrophoresis and electrofocalization examination of gamma globulins of the cerebrospinal fluid].[脑脊液γ球蛋白的琼脂电泳与电聚焦检查的比较结果]
Acta Neurol Belg. 1972 Jul-Aug;72(4):226-34.
10
[Quantitative simultaneous immunoelectrophoresis. A micromethod for the simultaneous determination of all serum proteins, including the immunoglobulins].
Z Klin Chem Klin Biochem. 1971 May;9(3):224-8.