Hjelle B, Chavez-Giles F, Torrez-Martinez N, Yamada T, Sarisky J, Ascher M, Jenison S
Department of Pathology, University of New Mexico School of Medicine, Albuquerque 87131-5301.
J Gen Virol. 1994 Nov;75 ( Pt 11):2881-8. doi: 10.1099/0022-1317-75-11-2881.
A newly identified hantavirus, tentatively called Four Corners virus (FCV), was found to be the aetiological agent of a 1993 outbreak of hantavirus pulmonary syndrome (HPS) in the southwestern United States. Immunodominant epitopes of 43 and 31 amino acids were identified in the nucleocapsid protein and G1 glycoprotein, respectively. The G1 genes of different hantaviruses are highly divergent, suggesting that geographically diverse FCVs might fail to cross-react owing to antigenic drift. We now show that the immunodominant epitope of G1 is conserved among 18 FCVs from a broad geographical area, despite extensive nucleotide sequence heterogeneity. Antibodies from all 45 HPS patients, separated by more than 3000 km were shown to be reactive with the dominant G1 epitope. Evidence for limited cross-reactivity between the G1 antigen of a novel hantavirus of the cotton rat and that of FCV is presented.
一种新发现的汉坦病毒，暂称为四角病毒（FCV），被发现是1993年美国西南部汉坦病毒肺综合征（HPS）疫情的病原体。分别在核衣壳蛋白和G1糖蛋白中鉴定出了43个和31个氨基酸的免疫显性表位。不同汉坦病毒的G1基因高度不同，这表明地理上不同的FCV可能由于抗原漂移而无法发生交叉反应。我们现在表明，尽管核苷酸序列存在广泛的异质性，但G1的免疫显性表位在来自广泛地理区域的18种FCV中是保守的。来自相距3000多公里的所有45名HPS患者的抗体都显示与显性G1表位有反应。本文提供了棉鼠新型汉坦病毒的G1抗原与FCV的G1抗原之间有限交叉反应性的证据。
