Harakeh H, Bosley G S, Keihlbauch J A, Fields B S
Biology Department, American University of Beirut, Lebanon.
J Clin Microbiol. 1994 Dec;32(12):3046-8. doi: 10.1128/jcm.32.12.3046-3048.1994.
Three multiresistant serotype 6B Streptococcus pneumoniae strains were isolated from the middle ear fluids of children undergoing tympanostomy in Atlanta. Because multiresistant 6B pneumococci have been reported to spread from a single clone, the three isolates were compared with 13 other multiresistant 6B pneumococci by hybridization of endonuclease-restricted DNA fragments with a digoxigenin-labeled cDNA probe complementary to 16 and 23S rRNAs (ribotyping). The ear isolates were heterogeneous, whereas six of the other pneumococcal isolates were alike, indicating a need for additional studies to determine the possibility of clonal spread.
从亚特兰大接受鼓膜造孔术的儿童中耳液中分离出三株多重耐药6B型肺炎链球菌。由于据报道多重耐药6B型肺炎球菌是从单一克隆传播而来，通过用与16和23S rRNA互补的地高辛标记cDNA探针进行核酸内切酶限制性DNA片段杂交（核糖分型），将这三株分离株与其他13株多重耐药6B型肺炎球菌进行了比较。耳部分离株具有异质性，而其他六株肺炎球菌分离株则相似，这表明需要进行更多研究以确定克隆传播的可能性。
