Mennone A, Alvaro D, Cho W, Boyer J L
Department of Medicine and Liver Center, Yale University School of Medicine, New Haven, CT 06520-8019, USA.
Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6527-31. doi: 10.1073/pnas.92.14.6527.
Fragments of small interlobular bile ducts averaging 20 microns in diameter can be isolated from rat liver. These isolated bile duct units form luminal spaces that are impermeant to dextran-40 and expand in size when cultured in 10 microM forskolin for 24-48 hr. Secretion is Cl- and HCO3- dependent and is stimulated by forskolin > dibutyryl cAMP > secretion but not by dideoxyforskolin, as assessed by video imaging techniques. Secretin stimulates Cl-/HCO3- exchange activity, and intraluminal pH increases after forskolin administration. These studies establish that small polarized physiologically intact interlobular bile ducts can be isolated from rat liver. These isolated bile duct units should be useful preparations for assessing the transport properties of small bile duct segments, which are the primary site of injury in cholestatic liver disorders, known as "vanishing bile duct syndromes."
平均直径为20微米的小叶间小胆管片段可从大鼠肝脏中分离出来。这些分离出的胆管单位形成管腔空间，对葡聚糖-40不通透，并且在10微摩尔的福斯可林中培养24至48小时时大小会扩大。分泌依赖于氯离子和碳酸氢根离子，并且如通过视频成像技术所评估的，福斯可林对其刺激作用大于二丁酰环磷腺苷>分泌，但双脱氧福斯可林无刺激作用。促胰液素刺激氯离子/碳酸氢根离子交换活性，并且在给予福斯可林后管腔内pH值升高。这些研究证实，可从大鼠肝脏中分离出小的具有极化性且生理功能完整的小叶间胆管。这些分离出的胆管单位应是评估小胆管段转运特性的有用制剂，小胆管段是胆汁淤积性肝病（即“消失胆管综合征”）中损伤的主要部位。
