脱氧核糖核酸分子通过感受态肺炎球菌表面摄取过程中对钙的需求步骤。
Calcium-requiring step in the uptake of deoxyribonucleic acid molecules through the surface of competent pneumococci.
作者信息
Seto H, Tomasz A
出版信息
J Bacteriol. 1976 Jun;126(3):1113-8. doi: 10.1128/jb.126.3.1113-1118.1976.
Abstract
The conversion of surface-adsorbed deoxyribonucleic acid (DNA) molecules to a state in which they are inaccessible to exogenous deoxyribonuclease requires specifically calcium ions; magnesium ions cannot replace calcium ions. Virtually maximal levels of nuclease-resistant DNA binding and genetic transformation can be obtained in media free from magnesium and containing only calcium ions. It is suggested that the calcium-requiring process is the transport of DNA molecules across the plasma membrane. Magnesium ions stimulate both the loss of surface-adsorbed DNA to the medium and the extracellular degradation of DNA.
摘要
表面吸附的脱氧核糖核酸（DNA）分子转变为对外源脱氧核糖核酸酶不可接近的状态需要特定的钙离子；镁离子不能替代钙离子。在不含镁离子且仅含钙离子的培养基中，几乎可以获得最高水平的核酸酶抗性DNA结合和遗传转化。有人提出，需要钙离子的过程是DNA分子跨质膜的转运。镁离子既刺激表面吸附的DNA向培养基中的流失，也刺激DNA的细胞外降解。
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本文引用的文献
1
Initiation of bacterial transformation.细菌转化的起始
Nature. 1957 Jun 29;179(4574):1322-5. doi: 10.1038/1791322a0.
2
Calcium-dependent bacteriophage DNA infection.钙依赖性噬菌体DNA感染。
J Mol Biol. 1970 Oct 14;53(1):159-62. doi: 10.1016/0022-2836(70)90051-3.
3
Genetic transformation in Escherichia coli K12.大肠杆菌K12中的基因转化
Proc Natl Acad Sci U S A. 1973 Jan;70(1):84-7. doi: 10.1073/pnas.70.1.84.
4
Fate of transforming DNA following uptake by competent Bacillus subtilis. IV. The endwise attachment and uptake of transforming DNA.感受态枯草芽孢杆菌摄取转化DNA后的命运。IV. 转化DNA的末端附着与摄取
J Mol Biol. 1972 Feb 28;64(1):31-46. doi: 10.1016/0022-2836(72)90319-1.
5
Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.细菌中的非染色体抗生素抗性：R 因子 DNA 对大肠杆菌的遗传转化
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4. doi: 10.1073/pnas.69.8.2110.
6
Cellular metabolism in genetic transformation of pneumococci: requirement for protein synthesis during induction of competence.肺炎链球菌遗传转化中的细胞代谢：感受态诱导过程中蛋白质合成的需求
J Bacteriol. 1970 Mar;101(3):860-71. doi: 10.1128/jb.101.3.860-871.1970.
7
On the nature of the pneumococcal activator substance.关于肺炎球菌激活物质的性质
Proc Natl Acad Sci U S A. 1966 Jan;55(1):58-66. doi: 10.1073/pnas.55.1.58.
8
Model for the mechanism controlling the expression of competent state in Pneumococcus cultures.肺炎球菌培养物中感受态状态表达控制机制的模型。
J Bacteriol. 1966 Mar;91(3):1050-61. doi: 10.1128/jb.91.3.1050-1061.1966.
9
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J Bacteriol. 1972 Jan;109(1):285-91. doi: 10.1128/jb.109.1.285-291.1972.
10
Sensitivity of Escherichia coli to viral nucleic acid. 8. Idiosyncrasy of Ca2+-dependent competence for DNA.大肠杆菌对病毒核酸的敏感性。8. 依赖Ca2+的DNA感受态的特异性。
J Biochem. 1974 Apr;75(4):895-904. doi: 10.1093/oxfordjournals.jbchem.a130463.
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