Jyonouchi H, Sun S, Tomita Y, Gross M D
Department of Pediatrics, School of Medicine, University of Minnesota, Minneapolis 55455, USA.
J Nutr. 1995 Oct;125(10):2483-92. doi: 10.1093/jn/125.10.2483.
Astaxanthin, a carotenoid without vitamin A activity, enhances T-dependent antigen (Ag)-specific humoral immune responses. We examined carotenoid actions on T-helper (Th) cell activity in a direct manner with reconstitution experiments; spleen Th cells were replaced with Ag-specific Type 1 and Type 2 (Th1 and Th2) Th cell clones. The Ag for the Th1 and Th2 clones were pigeon cytochrome C and rabbit gamma-globulin, respectively. Astaxanthin and beta-carotene augmented the number of IgM antibody (Ab)-secreting cells when unprimed B cells were incubated with Th clones and stimulated with suboptimal doses of Ag specific for each Th clone. The number of IgG Ab-secreting cells were greater with use of in vivo primed B cells than with unprimed B cells in both Th clones. Astaxanthin but not beta-carotene augmented the number of IgG Ab-secreting cells when primed B cells and Th cell clones were stimulated with suboptimal doses of Ag specific for each Th clone. In the presence of optimal doses of Ag for each Th clone, neither carotenoid augmented the number of Ab-secreting cells. Astaxanthin and beta-carotene may enhance the actions of both Th1 and Th2 cells for humoral immune responses with suboptimal Ag challenges; certain carotenoids may help maintain Ag-mediated immune responses at optimal levels.
虾青素是一种不具有维生素A活性的类胡萝卜素，可增强T细胞依赖性抗原（Ag）特异性体液免疫反应。我们通过重组实验直接研究了类胡萝卜素对辅助性T（Th）细胞活性的作用；用Ag特异性1型和2型（Th1和Th2）Th细胞克隆替换脾Th细胞。Th1和Th2克隆的Ag分别是鸽细胞色素C和兔γ球蛋白。当未致敏的B细胞与Th克隆一起孵育并用对每个Th克隆特异的次优剂量Ag刺激时，虾青素和β-胡萝卜素增加了分泌IgM抗体（Ab）的细胞数量。在两个Th克隆中，使用体内致敏的B细胞时分泌IgG Ab的细胞数量比未致敏的B细胞更多。当用对每个Th克隆特异的次优剂量Ag刺激致敏的B细胞和Th细胞克隆时，虾青素而非β-胡萝卜素增加了分泌IgG Ab的细胞数量。在每个Th克隆存在最佳剂量Ag的情况下，两种类胡萝卜素均未增加分泌Ab的细胞数量。虾青素和β-胡萝卜素可能通过次优Ag刺激增强Th1和Th2细胞对体液免疫反应的作用；某些类胡萝卜素可能有助于将Ag介导的免疫反应维持在最佳水平。
