Department of Periodontology, College of Dentistry, University of Florida, Gainesville, FL, USA.
J Dent Res. 2012 Oct;91(10):927-33. doi: 10.1177/0022034512456039. Epub 2012 Aug 3.
The objective of this study was to characterize the subgingival microbiota of African-American children with Localized Aggressive Periodontitis (LAP). Fifty-one children were included. Subgingival plaque samples were taken from diseased (DD) and healthy sites (DH) in LAP and from healthy sites in HS and HC and analyzed by 16S rRNA-based microarrays. Aggregatibacter actinomycetemcomitans (Aa) was the only species found to be both more prevalent (OR = 8.3, p = 0.0025) and abundant (p < 0.01) in DD. Filifactor alocis (Fa) was also found to be more prevalent in DD (OR 2.31, CI 1.06-5.01, p = 0.03). Most prevalent species in healthy sites were Selenomonas spp, Veillonella spp, Streptococcus spp, Bergeyella sp, and Kingella oralis. Overall, Streptococcus spp, Campylobacter gracilis, Capnocytophaga granulosa, Haemophilus parainfluenzae, and Lautropia mirabilis were most abundant in healthy children, while Aa, Fa, Tannerella sp, Solobacterium moorei, Parvimonas micra, and Capnocytophaga sp were most abundant in LAP. Based on a comprehensive analysis with 16S rRNA-based microarrays, Aa was strongly associated and site-specific in LAP. In contrast, other species were found to be associated with healthy sites and individuals (ClinicalTrials.gov number CT01330719).
healthy site in healthy sibling (HS); healthy site in healthy control child (HC).
本研究的目的是描述非裔美国儿童局限性侵袭性牙周炎（LAP）患者的龈下微生物群。共纳入 51 名儿童。从 LAP 的患病部位（DD）和健康部位（DH）、HS 和 HC 的健康部位采集龈下菌斑样本，并通过 16S rRNA 微阵列进行分析。伴放线放线杆菌（Aa）是唯一一种既更普遍（OR=8.3，p=0.0025）又丰富（p<0.01）的物种在 DD 中发现。Filifactor alocis（Fa）也被发现更普遍存在于 DD（OR 2.31，CI 1.06-5.01，p=0.03）。健康部位最常见的物种是 Selenomonas spp、Veillonella spp、Streptococcus spp、Bergeyella sp 和 Kingella oralis。总体而言，Streptococcus spp、Campylobacter gracilis、Capnocytophaga granulosa、Haemophilus parainfluenzae 和 Lautropia mirabilis 在健康儿童中最为丰富，而 Aa、Fa、Tannerella sp、Solobacterium moorei、Parvimonas micra 和 Capnocytophaga sp 在 LAP 中最为丰富。基于基于 16S rRNA 微阵列的综合分析，Aa 与 LAP 密切相关且具有特定部位。相比之下，其他物种被发现与健康部位和个体有关（ClinicalTrials.gov 编号 CT01330719）。
健康同胞部位（HS）；健康对照儿童的健康部位（HC）。
