Thrane U, Hansen U
Department of Biotechnology, Technical University of Denmark, Lyngby.
Mycopathologia. 1995;129(3):183-90. doi: 10.1007/BF01103345.
Forty-one isolates of Fusarium sambucinum sensu lato were screened for production of secondary metabolites in agar cultures. Of 16 strains of F. sambucinum sensu stricto all but two strains produced diacetoxyscirpenol and two unidentified metabolites, TB1 and TB2 respectively. The two remaining F. sambucinum strains produced T-2 toxin, TB1 and TB2. Fusarium venenotum (6 strains) produced diacetoxyscirpenol and an unidentified metabolite BB. Fusarium torulosum (8 strains) produced wortmannin and antibiotic Y. The three species could be differentiated by their pattern of identified and unidentified metabolites detected by agar plug TLC combined with chemical data from HPLC-diode array detection of fungal extracts, and data on growth rates on potato sucrose agar and tannin sucrose agar.
对41株拟接骨木镰刀菌进行了琼脂培养中次生代谢产物产生情况的筛选。在16株狭义接骨木镰刀菌中，除两株外，其余菌株均产生二乙酰氧基雪腐镰刀菌烯醇以及两种未鉴定的代谢产物，分别为TB1和TB2。其余两株接骨木镰刀菌产生T-2毒素、TB1和TB2。剧毒镰刀菌（6株）产生二乙酰氧基雪腐镰刀菌烯醇和一种未鉴定的代谢产物BB。串珠镰刀菌（8株）产生渥曼青霉素和抗生素Y。通过琼脂块薄层层析检测到的已鉴定和未鉴定代谢产物模式，结合真菌提取物的高效液相色谱-二极管阵列检测化学数据，以及在马铃薯蔗糖琼脂和单宁蔗糖琼脂上的生长速率数据，可以区分这三个物种。
