Carter I W, Cloonan M J
Department of Microbiology, Prince of Wales Hospital, Randwick, NSW.
Pathology. 1995 Apr;27(2):161-4. doi: 10.1080/00313029500169792.
Three methods for the detection of human cytomegalovirus DNA using the polymerase chain reaction (PCR) were compared with and without a wax-mediated hot start. This process yielded a 10-fold increase in the sensitivity of the detection of specific DNA. The PCR method chosen as most suitable for subsequent testing, when applied to urine samples from patients with AIDS, gave a higher proportion of positive results than either the shell vial assay or conventional cell culture. On the basis of these results, further work is being carried out to evaluate the value of the PCR, when the results are expressed quantitatively, in the laboratory diagnosis of cytomegalovirus infection in patients with AIDS.
比较了三种使用聚合酶链反应（PCR）检测人巨细胞病毒DNA的方法，有无蜡介导的热启动。这一过程使特异性DNA检测的灵敏度提高了10倍。被选为最适合后续检测的PCR方法，应用于艾滋病患者的尿液样本时，阳性结果的比例高于空斑试验或传统细胞培养。基于这些结果，正在进一步开展工作，以评估当结果以定量方式表达时，PCR在艾滋病患者巨细胞病毒感染实验室诊断中的价值。
