Deininger M, Meyermann R, Schluesener H
Institute of Brain Research, University of Tübingen, Germany.
Acta Neuropathol. 1995;90(1):76-9. doi: 10.1007/BF00294462.
The bone morphogenetic proteins (BMPs) constitute a novel subfamily of the transforming growth factor type beta (TGF-beta) supergene family. Here we demonstrate, using polymerase chain reaction (PCR) BMP-4 and BMP-5 messages in RNA isolated from multiple sclerosis (MS) plaque tissue. This is the first demonstration of BMP expression in an inflammatory lesion in general, and in MS in particular. However, BMP-4 and BMP-5 messages could be detected in RNA isolated from a Morbus Creutzfeldt-Jakob (CJD) lesion. Even in normal brain, RNA expression of BMP-4, but not that of BMP-5, was detected. Therefore, BMP-5 gene expression seems to be associated with MS and CJD lesions, whereas the BMP-4 gene appears to be constitutively expressed in the human brain. As TGF-beta s and BMPs are regulators of regenerative processes and contribute to regulation of chemoattraction and local immunoreactivity, BMP-4 and BMP-5 might be involved in aspects of MS lesion formation unknown so far. PCR analysis of human cell lines demonstrate BMP-4 and BMP-5 expression in leukocytic cells, suggesting that infiltrating leukocytes contribute at least in part to BMP-4 and BMP-5 mRNAs of the MS plaque.
骨形态发生蛋白（BMPs）构成了转化生长因子β（TGF-β）超基因家族的一个新亚家族。在此，我们使用聚合酶链反应（PCR）在从多发性硬化症（MS）斑块组织分离的RNA中证实了BMP-4和BMP-5的信息。这是首次在一般炎症病变中，特别是在MS中证实BMP的表达。然而，在从克雅氏病（CJD）病变分离的RNA中也能检测到BMP-4和BMP-5的信息。即使在正常大脑中，也检测到了BMP-4的RNA表达，但未检测到BMP-5的表达。因此，BMP-5基因表达似乎与MS和CJD病变相关，而BMP-4基因似乎在人类大脑中持续表达。由于TGF-β和BMP是再生过程的调节因子，并有助于调节化学吸引和局部免疫反应，BMP-4和BMP-5可能参与了迄今为止未知的MS病变形成过程。对人类细胞系的PCR分析表明，白细胞中存在BMP-4和BMP-5的表达，这表明浸润的白细胞至少部分地促成了MS斑块中BMP-4和BMP-5的mRNA的产生。
