肺炎克雷伯菌中β-内酰胺酶过量产生的分子基础——普里布诺框中的点突变
Point mutation in the pribnow box, the molecular basis of beta-lactamase overproduction in Klebsiella oxytoca.
作者信息
Fournier B, Lu C Y, Lagrange P H, Krishnamoorthy R, Philippon A
机构信息
Laboratoire de Microbiologie, Hôpital Saint-Louis, Université Paris VII, France.
出版信息
Antimicrob Agents Chemother. 1995 Jun;39(6):1365-8. doi: 10.1128/AAC.39.6.1365.
Abstract
Klebsiella oxytoca mutants resistant to a variety of beta-lactams were obtained in vitro on aztreonam. Constitutive beta-lactamase production was much higher in the mutants than in the susceptible strains (75-fold). The only difference observed in these mutants compared with the susceptible strains were point mutations in the Pribnow box: a transversion (G-->T) in the first base for one mutant or a transition (G-->A) in the fifth base of the -10 consensus sequence for the other three mutants. The transcriptional output of the beta-lactamase gene (blaOXY) from the mutants was significantly higher than that of the blaOXY gene from the susceptible strains.
摘要
在体外通过氨曲南获得了对多种β-内酰胺耐药的产酸克雷伯菌突变体。这些突变体中组成型β-内酰胺酶的产生量比敏感菌株高得多（75倍）。与敏感菌株相比，在这些突变体中观察到的唯一差异是普里布诺框中的点突变：一个突变体在 -10 共有序列的第一个碱基处发生颠换（G→T），另外三个突变体在第五个碱基处发生转换（G→A）。突变体中β-内酰胺酶基因（blaOXY）的转录产量明显高于敏感菌株中blaOXY基因的转录产量。
相似文献
1
Point mutation in the pribnow box, the molecular basis of beta-lactamase overproduction in Klebsiella oxytoca.肺炎克雷伯菌中β-内酰胺酶过量产生的分子基础——普里布诺框中的点突变
Antimicrob Agents Chemother. 1995 Jun;39(6):1365-8. doi: 10.1128/AAC.39.6.1365.
2
Strength and regulation of the different promoters for chromosomal beta-lactamases of Klebsiella oxytoca.产酸克雷伯菌染色体β-内酰胺酶不同启动子的强度及调控
Antimicrob Agents Chemother. 1999 Apr;43(4):850-5. doi: 10.1128/AAC.43.4.850.
3
beta-lactamase gene promoters of 71 clinical strains of Klebsiella oxytoca.71株产酸克雷伯菌临床菌株的β-内酰胺酶基因启动子
Antimicrob Agents Chemother. 1996 Feb;40(2):460-3. doi: 10.1128/AAC.40.2.460.
4
Klebsiella oxytoca: resistance to aztreonam by overproduction of the chromosomally encoded beta-lactamase.产酸克雷伯菌：通过过量产生染色体编码的β-内酰胺酶对氨曲南耐药。
FEMS Microbiol Lett. 1994 Feb 1;116(1):31-6. doi: 10.1111/j.1574-6968.1994.tb06671.x.
5
Genetic characterization of resistance to extended-spectrum beta-lactams in Klebsiella oxytoca isolates recovered from patients with septicemia at hospitals in the Stockholm area.从斯德哥尔摩地区医院败血症患者中分离出的产酸克雷伯菌菌株对超广谱β-内酰胺类抗生素耐药性的基因特征分析。
Antimicrob Agents Chemother. 1999 May;43(5):1294-7. doi: 10.1128/AAC.43.5.1294.
6
Chromosomal beta-lactamase genes of Klebsiella oxytoca are divided into two main groups, blaOXY-1 and blaOXY-2.产酸克雷伯菌的染色体β-内酰胺酶基因分为两个主要组，blaOXY-1和blaOXY-2。
Antimicrob Agents Chemother. 1996 Feb;40(2):454-9. doi: 10.1128/AAC.40.2.454.
7
Identification of clinical isolates of indole-positive Klebsiella spp., including Klebsiella planticola, and a genetic and molecular analysis of their beta-lactamases.吲哚阳性克雷伯菌属临床分离株的鉴定，包括植生克雷伯菌，及其β-内酰胺酶的遗传和分子分析。
J Clin Microbiol. 1997 Sep;35(9):2365-9. doi: 10.1128/jcm.35.9.2365-2369.1997.
8
9
Neonatal intensive care unit outbreak caused by a strain of Klebsiella oxytoca resistant to aztreonam due to overproduction of chromosomal beta-lactamase.由一株因染色体β-内酰胺酶过度产生而对氨曲南耐药的产酸克雷伯菌引起的新生儿重症监护病房暴发。
J Hosp Infect. 2001 Aug;48(4):281-8. doi: 10.1053/jhin.2001.1018.
10
Mechanism of resistance and antibacterial susceptibility in extended-spectrum β-lactamase phenotype Klebsiella pneumoniae and Klebsiella oxytoca isolated between 2000 and 2010 in Japan.2000年至2010年期间在日本分离出的产超广谱β-内酰胺酶表型的肺炎克雷伯菌和产酸克雷伯菌的耐药机制及药敏情况
J Med Microbiol. 2015 May;64(Pt 5):538-543. doi: 10.1099/jmm.0.000057. Epub 2015 Mar 26.
引用本文的文献
1
Detection of ESBL-producing complex with VITEK 2 system and screening cutoffs for implementing confirmatory tests.使用VITEK 2系统检测产超广谱β-内酰胺酶复合物及实施确证试验的筛查临界值。
J Clin Microbiol. 2025 Jun 11;63(6):e0012825. doi: 10.1128/jcm.00128-25. Epub 2025 May 9.
2
Clinically relevant mutations in regulatory regions of metabolic genes facilitate early adaptation to ciprofloxacin in Escherichia coli.临床相关的代谢基因调控区域突变有助于大肠杆菌对环丙沙星的早期适应。
Nucleic Acids Res. 2024 Sep 23;52(17):10385-10399. doi: 10.1093/nar/gkae719.
3
Clinical emergence of a novel extended-spectrum variant deriving from the OXY-1 β-lactamase.新型 OXY-1 类β-内酰胺酶衍生扩展谱变异体的临床出现。
Eur J Clin Microbiol Infect Dis. 2024 Nov;43(11):2215-2219. doi: 10.1007/s10096-024-04922-8. Epub 2024 Aug 22.
4
Evaluation of Phenotypic Tests to Detect Extended-Spectrum β-Lactamase (ESBL)-Producing Klebsiella oxytoca Complex Strains.产超广谱β-内酰胺酶（ESBL）的产酸克雷伯菌复合菌株表型检测方法的评估。
J Clin Microbiol. 2023 Apr 20;61(4):e0170622. doi: 10.1128/jcm.01706-22. Epub 2023 Mar 13.
5
Epidemiology and genomic analysis of Klebsiella oxytoca from a single hospital network in Australia.澳大利亚单家医院网络中产酸克雷伯菌的流行病学和基因组分析。
BMC Infect Dis. 2022 Aug 24;22(1):704. doi: 10.1186/s12879-022-07687-7.
6
Transcriptional Organization of the Typhimurium Phage P22 ORFan Locus.鼠伤寒沙门氏菌噬菌体P22 孤儿基因座的转录组织
Int J Mol Sci. 2022 Jan 23;23(3):1253. doi: 10.3390/ijms23031253.
7
Klebsiella oxytoca Complex: Update on Taxonomy, Antimicrobial Resistance, and Virulence.产酸克雷伯菌复合体：分类学、抗菌耐药性和毒力的最新研究进展。
Clin Microbiol Rev. 2022 Jan 19;35(1):e0000621. doi: 10.1128/CMR.00006-21. Epub 2021 Dec 1.
8
Variation in Accessory Genes Within the Species Complex Delineates Monophyletic Members and Simplifies Coherent Genotyping.物种复合体中辅助基因的变异描绘了单系成员并简化了连贯的基因分型。
Front Microbiol. 2021 Jul 2;12:692453. doi: 10.3389/fmicb.2021.692453. eCollection 2021.
9
Characterization of Piperacillin/Tazobactam-Resistant Klebsiella oxytoca Recovered from a Nosocomial Outbreak.从医院感染暴发中分离出的耐哌拉西林/他唑巴坦产酸克雷伯菌的特征分析
PLoS One. 2015 Nov 5;10(11):e0142366. doi: 10.1371/journal.pone.0142366. eCollection 2015.
10
Comparative genomics of an IncA/C multidrug resistance plasmid from Escherichia coli and Klebsiella isolates from intensive care unit patients and the utility of whole-genome sequencing in health care settings.来自重症监护病房患者的大肠杆菌和克雷伯菌分离株中IncA/C多药耐药质粒的比较基因组学以及全基因组测序在医疗环境中的应用
Antimicrob Agents Chemother. 2014 Aug;58(8):4814-25. doi: 10.1128/AAC.02573-14. Epub 2014 Jun 9.
本文引用的文献
1
Repressor binding to a regulatory site in the DNA coding sequence is sufficient to confer transcriptional regulation of the vir-repressed genes (vrg genes) in Bordetella pertussis.阻遏物与百日咳博德特氏菌中DNA编码序列的调控位点结合足以赋予对受vir基因阻遏的基因（vrg基因）的转录调控。
J Bacteriol. 1993 Jan;175(2):519-27. doi: 10.1128/jb.175.2.519-527.1993.
2
Klebsiella oxytoca: resistance to aztreonam by overproduction of the chromosomally encoded beta-lactamase.产酸克雷伯菌：通过过量产生染色体编码的β-内酰胺酶对氨曲南耐药。
FEMS Microbiol Lett. 1994 Feb 1;116(1):31-6. doi: 10.1111/j.1574-6968.1994.tb06671.x.
3
4
Requirement for an upstream element for optimal transcription of a bacterial tRNA gene.细菌tRNA基因最佳转录对上游元件的需求。
Nature. 1983;305(5931):248-50. doi: 10.1038/305248a0.
5
Compilation and analysis of Escherichia coli promoter DNA sequences.大肠杆菌启动子DNA序列的汇编与分析
Nucleic Acids Res. 1983 Apr 25;11(8):2237-55. doi: 10.1093/nar/11.8.2237.
6
Expression of malT, the regulator gene of the maltose region in Escherichia coli, is limited both at transcription and translation.大肠杆菌中麦芽糖区域调节基因malT的表达在转录和翻译水平上均受到限制。
EMBO J. 1982;1(3):369-74. doi: 10.1002/j.1460-2075.1982.tb01176.x.
7
Insertion of IS2 creates a novel ampC promoter in Escherichia coli.IS2的插入在大肠杆菌中产生了一个新的ampC启动子。
Cell. 1983 Mar;32(3):809-16. doi: 10.1016/0092-8674(83)90067-3.
8
Selection of multiple antibiotic resistance by quinolones, beta-lactams, and aminoglycosides with special reference to cross-resistance between unrelated drug classes.喹诺酮类、β-内酰胺类和氨基糖苷类药物对多重抗生素耐药性的选择，特别提及不相关药物类别之间的交叉耐药性。
Antimicrob Agents Chemother. 1984 Dec;26(6):797-801. doi: 10.1128/AAC.26.6.797.
9
[Klebsiella oxytoca beta-lactamases: study of their action on 3d-generation cephalosporins].[产酸克雷伯菌β-内酰胺酶：其对第三代头孢菌素作用的研究]
Pathol Biol (Paris). 1986 Jun;34(5 Pt 2):611-5.
10
Contribution of chromosomal beta-lactamases to beta-lactam resistance in enterobacteria.染色体β-内酰胺酶在肠杆菌科细菌对β-内酰胺类抗生素耐药性中的作用。
Rev Infect Dis. 1986 Jul-Aug;8 Suppl 3:S292-304. doi: 10.1093/clinids/8.supplement_3.s292.
Zotero 插件