Adegboye D S, Hallbur P G, Cavanaugh D L, Werdin R E, Chase C C, Miskimins D W, Rosenbusch R F
Veterinary Medical Research Institute, Iowa State University, Ames 50011-1240, USA.
J Vet Diagn Invest. 1995 Jul;7(3):333-7. doi: 10.1177/104063879500700306.
Out of 45 cases of fatal chronic pneumonia in calves examined for Mycoplasma bovis infection from February to July 1994, 11 cases with pulmonary abscesses that were culture positive for M. bovis were encountered. The cases were studied in detail using a recently developed monoclonal antibody-based immunoperoxidase technique. Mycoplasma bovis organisms were detected in specific locations at all stages of abscessation observed. In bronchioles or terminal airways within which abscesses developed, M. bovis was located at the epithelial surface and in close association with infiltrating neutrophils and macrophages. Abscessed airways that had lost the epithelium were encapsulated and were seen as coagulative necrotic foci that stained intensely for M. bovis, especially at the periphery. Some foci stained weakly and such might have been resolving lesions. Mycoplasma bovis was also demonstrated at sites of mild mononuclear cell infiltration in the livers and kidneys of 2 calves. The mycoplasma was detected within bile ducts in the liver and in the tubular epithelium of the kidney. Abscesses not staining for M. bovis, presumably caused by other pathogens, were seen concurrently with M. bovis-associated abscesses in some lungs. Thirteen other M. bovis-positive cases showed no abscesses, possibly indicating heterogeneity among M. bovis strains. Three other cases with abscesses were negative for M. bovis by culture and immunoperoxidase staining. The monoclonal antibody-based immunohistochemical technique is efficient for specific detection of M. bovis in cases of enzootic pneumonia of calves with or without abscessation. Mycoplasma bovis is implicated in the pathogenesis of lung abscesses in some calves.
1994年2月至7月间，对45例死于慢性肺炎的犊牛进行牛支原体感染检测，发现11例肺部脓肿病例的牛支原体培养呈阳性。采用最近开发的基于单克隆抗体的免疫过氧化物酶技术对这些病例进行了详细研究。在观察到的脓肿形成的各个阶段，均在特定位置检测到牛支原体。在形成脓肿的细支气管或终末气道中，牛支原体位于上皮表面，并与浸润的中性粒细胞和巨噬细胞紧密相关。失去上皮的脓肿气道被包裹，呈现为凝固性坏死灶，牛支原体染色强烈，尤其是在周边部位。一些病灶染色较弱，可能是正在消散的病变。在2头犊牛的肝脏和肾脏轻度单核细胞浸润部位也检测到了牛支原体。在肝脏的胆管和肾脏的肾小管上皮内检测到了支原体。在一些肺中，与牛支原体相关的脓肿同时可见由其他病原体引起的不被牛支原体染色的脓肿。另外13例牛支原体阳性病例未出现脓肿，这可能表明牛支原体菌株存在异质性。另外3例有脓肿的病例经培养和免疫过氧化物酶染色牛支原体呈阴性。基于单克隆抗体的免疫组织化学技术可有效特异性检测有或无脓肿形成的犊牛地方流行性肺炎病例中的牛支原体。牛支原体与一些犊牛肺脓肿的发病机制有关。
