Fujishima H, Takeuchi T, Shinozaki N, Saito I, Tsubota K
Department of Opthalmology, Tokyo Dental College, Chiba, Japan.
Clin Exp Immunol. 1995 Nov;102(2):395-8. doi: 10.1111/j.1365-2249.1995.tb03796.x.
To elucidate the mechanism of ocular surface allergic disease, we focused on IL-4, which is one of the key factors in regulating IgE production, and thus determined the concentration of IL-4 in tears. IL-4 concentration was determined in the tears of 15 patients with seasonal allergic conjunctivitis, 15 vernal keratoconjunctivitis (VKC), 10 giant papillary conjunctivitis (GPC), 10 patients with non-allergic conjunctivitis and post-cataract surgical conjunctivitis as intermediate conjunctivitis, and 10 normal subjects using a highly sensitive sandwich ELISA. The mean level of IL-4 in normal controls was low, and seasonal allergic conjunctivitis, VKC and GPC showed a significant elevation (P < 0.05), respectively. IL-4 of VKC and GPC were also significantly higher than allergic conjunctivitis, and non-allergic conjunctivitis and post-cataract surgical conjunctivitis were not higher than normal. These results raise the possibility that the increased level of IL-4 in tears could play a role in allergic disease and its severity in patients.
为阐明眼表过敏性疾病的机制，我们聚焦于白细胞介素-4（IL-4），它是调节免疫球蛋白E（IgE）产生的关键因素之一，因此测定了泪液中IL-4的浓度。采用高灵敏度夹心酶联免疫吸附测定法（ELISA），对15例季节性过敏性结膜炎患者、15例春季角结膜炎（VKC）患者、10例巨乳头性结膜炎（GPC）患者、10例非过敏性结膜炎患者以及作为中度结膜炎的白内障术后结膜炎患者的泪液，以及10名正常受试者的泪液进行了IL-4浓度测定。正常对照组中IL-4的平均水平较低，季节性过敏性结膜炎、VKC和GPC患者的IL-4水平分别显著升高（P < 0.05）。VKC和GPC患者的IL-4水平也显著高于过敏性结膜炎患者，而非过敏性结膜炎和白内障术后结膜炎患者的IL-4水平不高于正常水平。这些结果提示，泪液中IL-4水平升高可能在患者的过敏性疾病及其严重程度中起作用。
