[一种用于定量血清中白细胞介素-4的方法（夹心酶联免疫吸附测定法）及特应性皮炎患者的白细胞介素-4水平]
[A method for the quantification of interleukin-4 in serum (sandwich ELISA) and IL-4 levels in patients with atopic dermatitis].
作者信息
Kasamatsu M, Tsuji T, Miura M
机构信息
Department of Dermatology, Nagoya City University Medical School.
出版信息
Arerugi. 1993 Jul;42(7):878-82.
PMID:8363440
Abstract
We have developed a sandwich enzyme-linked immunosorbent assay (ELISA) using chemiluminescent enzyme substrate AMPPD for the quantification of interleukin 4 (IL-4) in serum. IL-4 in serum could be measured in concentrations from 0.17 pg/ml. The IL-4 levels in patients with atopic dermatitis were significantly higher than those in normal controls.
摘要
我们开发了一种夹心酶联免疫吸附测定法（ELISA），使用化学发光酶底物AMPPD来定量血清中的白细胞介素4（IL-4）。血清中的IL-4浓度可低至0.17 pg/ml进行测量。特应性皮炎患者的IL-4水平显著高于正常对照组。
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