Sherburne R, Taylor D E
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada.
Infect Immun. 1995 Dec;63(12):4564-8. doi: 10.1128/iai.63.12.4564-4568.1995.
Monoclonal antibodies (MAbs) specific for Lewis X (Lex) reacted with whole cells of Helicobacter pylori NCTC11637, UA799, UA802, UA825, UA861, UA1182, and UA1206 in immunoelectron microscopy and enzyme-linked immunosorbent assay (ELISA) experiments. These MAbs have documented specificity to Lex, whereas MAbs for Lea and Leb were negative in both immunoelectron microscopy and ELISA. H. pylori coccoid forms also reacted with the MAbs, whereas the flagellum lacking the sheath showed no reactivity. The Lex structures were associated with membrane fractions in the ELISA experiments, and silver-stained sodium dodecyl sulfate-polyacrylamide gels confirmed the presence of lipopolysaccharides which reacted with the MAbs in immunoblots. Serum from an H. pylori-infected individual contained immunoglobulins which blocked the binding of the Lex MAbs, indicating that part of the host immune response to H. pylori is to the Lex structure. The ability of this gastric pathogen to mimic an oncofetal antigen (self) could explain the down regulation of anti-H. pylori T-cell response seen in H. pylori-infected individuals.
针对Lewis X（Lex）的单克隆抗体（MAb）在免疫电子显微镜和酶联免疫吸附测定（ELISA）实验中与幽门螺杆菌NCTC11637、UA799、UA802、UA825、UA861、UA1182和UA1206的全细胞发生反应。这些单克隆抗体已证明对Lex具有特异性，而针对Lea和Leb的单克隆抗体在免疫电子显微镜和ELISA中均为阴性。幽门螺杆菌的球状体形式也与这些单克隆抗体发生反应，而缺乏鞘的鞭毛则无反应性。在ELISA实验中，Lex结构与膜组分相关，十二烷基硫酸钠 - 聚丙烯酰胺凝胶银染证实了在免疫印迹中与单克隆抗体发生反应的脂多糖的存在。幽门螺杆菌感染个体的血清中含有可阻断Lex单克隆抗体结合的免疫球蛋白，表明宿主对幽门螺杆菌的部分免疫反应是针对Lex结构的。这种胃部病原体模拟癌胚抗原（自身）的能力可以解释在幽门螺杆菌感染个体中观察到的抗幽门螺杆菌T细胞反应的下调。
