Molnár I, Kaczur V, Boros A, Krajczár G, Balázs C
3rd Department of Medicine, Kenézy County Hospital, Debrecen, Hungary.
J Endocrinol Invest. 1995 Jun;18(6):408-14. doi: 10.1007/BF03349737.
The presence of IgA autoantibodies against human eye muscle was investigated in 40 patients with Graves' disease (33 had ophthalmopathy). IgA anti-eye muscle antibodies could be demonstrated in sera of patients using western blotting and immunohistochemical methods. For the detection of sera possessing autoantibodies against eye muscle antigen the indirect immunosorbent assay had been used. IgA anti-eye muscle antibodies could be demonstrated in 25 cases and IgG types in 16 cases out of 40 patients. These anti-muscle autoantibodies were associated with eye muscle rather than skeletal muscle, the number of positive cases with the latter being 5/40 for IgG and 2/40 for IgA. Immunoreactive bands of IgA autoantibodies against eye cytosol were found at 84, 64, 45, 40 and 25-23 kDa in 22, 2, 16, 2 and 18 cases, respectively. A difference was observed in the staining of IgG and IgA types of autoantibodies by immunohistochemical analysis of eye muscle tissue. The IgA anti-eye muscle antibodies reacted with muscle fibers and the IgG types showed staining on endomysium. No sera of Graves' disease patients gave staining on skeletal muscle tissue. The results supported the presence of IgA anti-human eye muscle antibodies in patients with Graves' ophthalmopathy, which might play a relevant role in the development of eye disease.
对40例格雷夫斯病患者（其中33例有眼病）进行了抗人眼肌IgA自身抗体的检测。采用蛋白质印迹法和免疫组织化学方法可在患者血清中检测到IgA抗眼肌抗体。为检测含有抗眼肌抗原自身抗体的血清，采用了间接免疫吸附试验。40例患者中，25例可检测到IgA抗眼肌抗体，16例可检测到IgG型抗体。这些抗肌肉自身抗体与眼肌相关，而非骨骼肌，后者IgG阳性病例数为5/40，IgA阳性病例数为2/40。针对眼细胞溶质的IgA自身抗体的免疫反应条带在22例、2例、16例、2例和18例中分别出现在84、64、45、40和25 - 23 kDa处。通过对眼肌组织的免疫组织化学分析观察到IgG和IgA类型自身抗体染色存在差异。IgA抗眼肌抗体与肌纤维反应，IgG型抗体在内膜上显示染色。格雷夫斯病患者的血清在骨骼肌组织上均未出现染色。结果支持格雷夫斯眼病患者存在IgA抗人眼肌抗体，其可能在眼病发展中起相关作用。
