Torabinejad M, Hong C U, Pitt Ford T R, Kettering J D
Department of Endodontics, School of Dentistry, Loma Linda University, California, USA.
J Endod. 1995 Aug;21(8):403-6. doi: 10.1016/s0099-2399(06)80824-1.
In addition to having good sealing ability, root end filling materials should "ideally" have some antibacterial activity to prevent bacterial growth. This investigation compared the antibacterial effects of amalgam, zinc oxide-eugenol, Super EBA and a mineral trioxide aggregate on nine facultative bacteria Streptococcus fecalis, Streptococcus mitis, Streptococcus mutans, Streptococcus salivarius, Lactobacillus species, Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, and Escherichia coli B and seven strict anaerobic bacteria, Prevotella (Bacteroides) buccae, Bacteroides fragilis, Prevotella (Bacteroides) intermedia, Prevotella (Bacteroides) melaninogenica, Fusobacterium necrophorum, Fusobacterium nucleatum, and Peptostreptococcus anaerobius. After growing these bacteria on solid media, freshly mixed and 24-h set test materials were placed on the surface of these inoculated media and incubated in the appropriate atmosphere for 24 to 48 h at 37 degrees C. Impregnated discs with the Super EBA liquid were used as positive controls. The antibacterial effects of each material were measured in millimeters and the data were analyzed using one-way and two-way analysis of variance and Scheffé tests to determine the statistical differences between the antibacterial effects of the test materials. Impregnated discs with Super EBA liquid caused varying degrees of growth inhibition for both facultative and strict anaerobic bacteria. Both types of amalgam had no antibacterial effect against any of the bacteria tested in this study. Mineral trioxide aggregate had an antibacterial effect on some of the facultative bacteria and no effect on any of the strict anaerobic bacteria. Zinc oxide-eugenol and Super EBA pastes had some antibacterial effects on both types of bacteria tested.(ABSTRACT TRUNCATED AT 250 WORDS)
除了具有良好的封闭能力外，理想情况下，根尖充填材料应具有一定的抗菌活性以防止细菌生长。本研究比较了汞合金、氧化锌丁香油、超级EBA和矿物三氧化物凝聚体对9种兼性菌（粪肠球菌、缓症链球菌、变形链球菌、唾液链球菌、乳杆菌属、金黄色葡萄球菌、表皮葡萄球菌、枯草芽孢杆菌和大肠杆菌B）以及7种严格厌氧菌（颊普雷沃菌（拟杆菌属）、脆弱拟杆菌、中间普雷沃菌（拟杆菌属）、产黑色素普雷沃菌（拟杆菌属）、坏死梭杆菌、具核梭杆菌和厌氧消化链球菌）的抗菌效果。在固体培养基上培养这些细菌后，将新鲜混合的和24小时凝固后的测试材料置于这些接种培养基的表面，并在37℃的适宜环境中孵育24至48小时。用超级EBA液体浸泡的圆片用作阳性对照。每种材料的抗菌效果以毫米为单位进行测量，并使用单向和双向方差分析以及谢费检验对数据进行分析，以确定测试材料抗菌效果之间的统计学差异。用超级EBA液体浸泡的圆片对兼性菌和严格厌氧菌均产生不同程度的生长抑制。两种类型的汞合金对本研究中测试的任何细菌均无抗菌作用。矿物三氧化物凝聚体对一些兼性菌有抗菌作用，对任何严格厌氧菌均无作用。氧化锌丁香油和超级EBA糊剂对所测试的两种类型细菌均有一定的抗菌作用。（摘要截短为250字）
