Sullivan J T, Fraser K M, Fleming S B, Robinson A J, Mercer A A
Health Research Council Virus Research Unit, University of Otago, Dunedin, New Zealand.
Virus Genes. 1995 Feb;9(3):277-82. doi: 10.1007/BF01702883.
A 1608 bp region located approximately 5.0 kb from the left end of the orf virus (OV) genome (strain NZ2) was sequenced. The sequence revealed a single open reading frame designated G1L. The predicted amino acid sequence of G1L contained eight ankyrinlike repeat sequences. Transcriptional analysis of G1L showed it was transcribed towards the genome terminus during the early phase of infection. S1 nuclease and primer extension analyses showed that the transcriptional start site of the gene was located a short distance downstream from an A + T-rich sequence similar to a vaccinia virus early promoter.
对位于orf病毒（OV）基因组（NZ2株）左端约5.0 kb处的一段1608 bp区域进行了测序。该序列显示有一个单一的开放阅读框，命名为G1L。G1L的预测氨基酸序列包含8个锚蛋白样重复序列。对G1L的转录分析表明，在感染早期它朝着基因组末端转录。S1核酸酶和引物延伸分析表明，该基因的转录起始位点位于一个类似于痘苗病毒早期启动子的富含A + T序列下游的短距离处。
