Kempe M, Glad M, Mosbach K
University of Lund, Sweden.
J Mol Recognit. 1995 Jan-Apr;8(1-2):35-9. doi: 10.1002/jmr.300080106.
An adsorbent showing enhanced selectivity for the enzyme RNase A was prepared by a surface imprinting procedure based on metal coordination. A metal chelating monomer, N-(4-vinyl)-benzyl iminodiacetic acid, was polymerized onto methacrylate-derivatized silica particles in the presence of RNase A and metal ions. Lysozyme and RNase A were separated on the adsorbent used as stationary phase in high-performance liquid chromatography.
通过基于金属配位的表面印迹法制备了一种对核糖核酸酶A（RNase A）具有增强选择性的吸附剂。在RNase A和金属离子存在的情况下，将金属螯合单体N-(4-乙烯基)苄基亚氨基二乙酸聚合到甲基丙烯酸酯衍生的硅胶颗粒上。在高效液相色谱中，溶菌酶和RNase A在用作固定相的吸附剂上得以分离。
