Kuramoto T, Mashimo T, Serikawa T
Institute of Laboratory Animals, Faculty of Medicine, Kyoto University, Japan.
Exp Anim. 1995 Apr;44(2):119-25. doi: 10.1538/expanim.44.119.
In order to develop genetic markers in rats, arbitrarily primed polymerase chain reaction (AP-PCR) was performed with single primers originally designed for microsatellite loci, instead of primers with short-sized and arbitrary nucleotide sequences. Each primer generated reliable and reproducible segments under optimal conditions. The fourteen amplification products have been successfully mapped to rat chromosomes by either linkage analysis using backcross progeny or chromosomal assignment with somatic cell hybrids. All of the loci were located on different chromosomes from those of the microsatellite loci, suggesting that sequence-specifically designed single primers can produce anonymous segments of genomic DNA showing polymorphisms. These markers should contribute to finding linkages for traits of interest in rats.
为了在大鼠中开发遗传标记，使用最初为微卫星位点设计的单引物进行任意引物聚合酶链反应（AP-PCR），而不是使用短尺寸和任意核苷酸序列的引物。在最佳条件下，每个引物都能产生可靠且可重复的片段。通过使用回交后代进行连锁分析或使用体细胞杂种进行染色体定位，已成功将这14个扩增产物定位到大鼠染色体上。所有这些位点都位于与微卫星位点不同的染色体上，这表明序列特异性设计的单引物可以产生显示多态性的基因组DNA的匿名片段。这些标记应该有助于找到大鼠中感兴趣性状的连锁关系。
