通过随机引物聚合酶链反应鉴定29个大鼠遗传标记
Identification of 29 rat genetic markers by arbitrarily primed polymerase chain reaction.
作者信息
Canzian F, Ushijima T, Toyota M, Hosoya Y, Sugimura T, Nagao M
机构信息
Carcinogenesis Division, National Cancer Center Research Institute, Tokyo.
出版信息
Jpn J Cancer Res. 1996 Jul;87(7):669-75. doi: 10.1111/j.1349-7006.1996.tb00275.x.
The number of genetic markers for the rat is still limited, in spite of its wide use in cancer research. To facilitate accurate mapping of both established and novel rat genetic markers, we constructed a linkage map by genotyping 105 F2 rats from ACI/N (ACI) and BUF/Nac (BUF) crosses. This map consists of 120 genetic markers that had been previously reported, mainly by two research groups, but had not been integrated. To find new genetic markers, the arbitrarily primed polymerase chain reaction (AP-PCR) was applied to detect polymorphic bands between ACI and BUF rats. After testing 56 single primers and 12 combinations of primers, we found 36 bands produced by 16 single primers and two combinations to be reliably polymorphic between ACI and BUF rats. The 36 bands were typed in the 105 F2 rats, and 29 of them could be linkage-mapped. AP-PCR is thus useful to detect new genetic markers in laboratory strains of rats.
尽管大鼠在癌症研究中得到广泛应用，但其遗传标记的数量仍然有限。为了便于准确绘制已有的和新的大鼠遗传标记图谱，我们通过对来自ACI/N（ACI）和BUF/Nac（BUF）杂交的105只F2大鼠进行基因分型构建了一个连锁图谱。该图谱由120个先前报道的遗传标记组成，主要来自两个研究小组，但尚未整合。为了寻找新的遗传标记，应用任意引物聚合酶链反应（AP-PCR）来检测ACI和BUF大鼠之间的多态性条带。在测试了56个单引物和12个引物组合后，我们发现由16个单引物和两个组合产生的36条带在ACI和BUF大鼠之间具有可靠的多态性。在105只F2大鼠中对这36条带进行分型，其中29条可以进行连锁定位。因此，AP-PCR可用于检测大鼠实验室品系中的新遗传标记。
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